TY - JOUR
T1 - Harmonizing light transmission aggregometry in the Netherlands by implementation of the SSC-ISTH guideline
AU - Munnix, I. C.A.
AU - Van Oerle, R.
AU - Verhezen, P.
AU - Kuijper, P.
AU - Hackeng, C. M.
AU - Hopman-Kerkhoff, H. I.J.
AU - Hudig, F.
AU - Van De Kerkhof, D.
AU - Leyte, A.
AU - De Maat, M. P.M.
AU - Oude Elferink, R. F.M.
AU - Ruinemans-Koerts, J.
AU - Schoorl, M.
AU - Slomp, J.
AU - Soons, H.
AU - Stroobants, A.
AU - Van Wijk, E.
AU - Henskens, Y. M.C.
N1 - Funding Information:
The authors thank C. Klopper, S. Busscher, H. Nieman-Teunis, T. de Boer, J. van Empel, N. van Dijk and C. van den Nieuwenhof for performing the LTA.
Publisher Copyright:
© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.
PY - 2021
Y1 - 2021
N2 - Light transmission aggregometry (LTA) is considered the gold standard method for evaluation of platelet function. However, there are a lot of variation in protocols (pre-analytical procedures and agonist concentrations) and results. The aim of our study was to establish a national LTA protocol, to investigate the effect of standardization and to define national reference values for LTA. The SSC guideline was used as base for a national procedure. Almost all recommendations of the SSC were followed e.g. no adjustment of PRP, citrate concentration of 109 mM, 21 needle gauge, fasting, resting time for whole blood and PRP, centrifugation time, speed and agonists concentrations. LTA of healthy volunteers was measured in a total of 16 hospitals with 5 hospitals before and after standardization. Results of more than 120 healthy volunteers (maximum aggregation %) were collected, with participating laboratories using 4 different analyzers with different reagents. Use of low agonist concentrations showed high variation before and after standardization, with the exception of collagen. For most high agonist concentrations (ADP, collagen, ristocetin, epinephrine and arachidonic acid) variability in healthy subjects decreased after standardization. We can conclude that a standardized Dutch protocol for LTA, based on the SSC guideline, does not result in smaller variability in healthy volunteers for all agonist concentrations.
AB - Light transmission aggregometry (LTA) is considered the gold standard method for evaluation of platelet function. However, there are a lot of variation in protocols (pre-analytical procedures and agonist concentrations) and results. The aim of our study was to establish a national LTA protocol, to investigate the effect of standardization and to define national reference values for LTA. The SSC guideline was used as base for a national procedure. Almost all recommendations of the SSC were followed e.g. no adjustment of PRP, citrate concentration of 109 mM, 21 needle gauge, fasting, resting time for whole blood and PRP, centrifugation time, speed and agonists concentrations. LTA of healthy volunteers was measured in a total of 16 hospitals with 5 hospitals before and after standardization. Results of more than 120 healthy volunteers (maximum aggregation %) were collected, with participating laboratories using 4 different analyzers with different reagents. Use of low agonist concentrations showed high variation before and after standardization, with the exception of collagen. For most high agonist concentrations (ADP, collagen, ristocetin, epinephrine and arachidonic acid) variability in healthy subjects decreased after standardization. We can conclude that a standardized Dutch protocol for LTA, based on the SSC guideline, does not result in smaller variability in healthy volunteers for all agonist concentrations.
UR - http://www.scopus.com/inward/record.url?scp=85087038777&partnerID=8YFLogxK
U2 - 10.1080/09537104.2020.1771549
DO - 10.1080/09537104.2020.1771549
M3 - Article
C2 - 32522065
AN - SCOPUS:85087038777
SN - 0953-7104
VL - 32
SP - 516
EP - 523
JO - Platelets
JF - Platelets
IS - 4
ER -