Headplate Installation and Craniotomy for Awake In VivoElectrophysiological Recordings or Two-Photon Imaging of the Mouse Inferior Colliculus

Blom Kraakman, Sofja Solovjova, J. Gerard G. Borst, Aaron B. Wong*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The inferior colliculus (IC) is an important processing center in the auditory system, which also receives non-auditory sensory input. The IC consists of several subnuclei whose functional role in (non-) auditory processing and plastic response properties are best approached by studying awake animals, preferably in a longitudinal fashion. The increasing use of mice in auditory research, the availability of genetic models, and the superficial location of the IC in the mouse have made it an attractive species for studying IC function. Here, we describe a protocol for exposing the mouse IC for up to a few weeks for in vivo imaging or electrophysiology in a stable manner. This method allows for a broader sampling of the IC while maintaining the brain surface in good quality and without reopening the craniotomy. Moreover, as it is adaptable for both electrophysiological recordings of the entire IC and imaging of the dorsal IC surface, it can be applied to answer a multitude of questions.

Original languageEnglish
Article number14902
JournalBio-Protocol
Volume13
Issue number24
DOIs
Publication statusPublished - 20 Dec 2023

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Publisher Copyright: © 2023 The Authors.

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