TY - JOUR
T1 - Heterologous up-regulation of the 1,25-dihydroxyvitamin D3 receptor by parathyroid hormone (PTH) and PTH-like peptide in osteoblast-like cells
AU - Pols, H. A.P.
AU - van Leeuwen, J. P.T.M.
AU - Schilte, J. P.
AU - Visser, T. J.
AU - Birkenhäger, J. C.
PY - 1988/10/14
Y1 - 1988/10/14
N2 - 1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) receptor content in cultured osteogenic sarcoma cells (UMR-106) was found to be increased after treatment with both bovine and human PTH and human PTH-like peptide (hPLP). The dose dependent increase of receptors was preceded by a dose dependent stimulation of cAMP production. This suggests a role for cAMP as mediator of the PTH- and hPLP-induced 1,25-(OH)2D3 receptor up-regulation. Furthermore, evidence was obtained that new mRNA and de novo receptor synthesis is involved in this heterologous 1,25-(OH)2D3 receptor up-regulation.
AB - 1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) receptor content in cultured osteogenic sarcoma cells (UMR-106) was found to be increased after treatment with both bovine and human PTH and human PTH-like peptide (hPLP). The dose dependent increase of receptors was preceded by a dose dependent stimulation of cAMP production. This suggests a role for cAMP as mediator of the PTH- and hPLP-induced 1,25-(OH)2D3 receptor up-regulation. Furthermore, evidence was obtained that new mRNA and de novo receptor synthesis is involved in this heterologous 1,25-(OH)2D3 receptor up-regulation.
UR - http://www.scopus.com/inward/record.url?scp=0024286303&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(88)80883-0
DO - 10.1016/S0006-291X(88)80883-0
M3 - Article
C2 - 2845985
AN - SCOPUS:0024286303
SN - 0006-291X
VL - 156
SP - 588
EP - 594
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -