Heterologous up-regulation of the 1,25-dihydroxyvitamin D3 receptor by parathyroid hormone (PTH) and PTH-like peptide in osteoblast-like cells

H. A.P. Pols; J. P.T.M. van Leeuwen; J. P. Schilte; T. J. Visser; J. C. Birkenhäger

doi:10.1016/S0006-291X(88)80883-0

Heterologous up-regulation of the 1,25-dihydroxyvitamin D₃ receptor by parathyroid hormone (PTH) and PTH-like peptide in osteoblast-like cells

H. A.P. Pols^*
, J. P.T.M. van Leeuwen
, J. P. Schilte
, T. J. Visser
, J. C. Birkenhäger

^*Corresponding author for this work

Internal Medicine

Erasmus University Rotterdam

Research output: Contribution to journal › Article › Academic › peer-review

46 Citations (Scopus)

Abstract

1,25-Dihydroxyvitamin D₃ (1,25-(OH)₂D₃) receptor content in cultured osteogenic sarcoma cells (UMR-106) was found to be increased after treatment with both bovine and human PTH and human PTH-like peptide (hPLP). The dose dependent increase of receptors was preceded by a dose dependent stimulation of cAMP production. This suggests a role for cAMP as mediator of the PTH- and hPLP-induced 1,25-(OH)₂D₃ receptor up-regulation. Furthermore, evidence was obtained that new mRNA and de novo receptor synthesis is involved in this heterologous 1,25-(OH)₂D₃ receptor up-regulation.

Original language	English
Pages (from-to)	588-594
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	156
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(88)80883-0
Publication status	Published - 14 Oct 1988

Access to Document

10.1016/S0006-291X(88)80883-0

Cite this

@article{5080976875034a9898367bf199bbea75,

title = "Heterologous up-regulation of the 1,25-dihydroxyvitamin D3 receptor by parathyroid hormone (PTH) and PTH-like peptide in osteoblast-like cells",

abstract = "1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) receptor content in cultured osteogenic sarcoma cells (UMR-106) was found to be increased after treatment with both bovine and human PTH and human PTH-like peptide (hPLP). The dose dependent increase of receptors was preceded by a dose dependent stimulation of cAMP production. This suggests a role for cAMP as mediator of the PTH- and hPLP-induced 1,25-(OH)2D3 receptor up-regulation. Furthermore, evidence was obtained that new mRNA and de novo receptor synthesis is involved in this heterologous 1,25-(OH)2D3 receptor up-regulation.",

author = "Pols, \{H. A.P.\} and \{van Leeuwen\}, \{J. P.T.M.\} and Schilte, \{J. P.\} and Visser, \{T. J.\} and Birkenh{\"a}ger, \{J. C.\}",

year = "1988",

month = oct,

day = "14",

doi = "10.1016/S0006-291X(88)80883-0",

language = "English",

volume = "156",

pages = "588--594",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier",

number = "1",

}

Heterologous up-regulation of the 1,25-dihydroxyvitamin D₃ receptor by parathyroid hormone (PTH) and PTH-like peptide in osteoblast-like cells. / Pols, H. A.P.; van Leeuwen, J. P.T.M.; Schilte, J. P. et al.
In: Biochemical and Biophysical Research Communications, Vol. 156, No. 1, 14.10.1988, p. 588-594.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Heterologous up-regulation of the 1,25-dihydroxyvitamin D3 receptor by parathyroid hormone (PTH) and PTH-like peptide in osteoblast-like cells

AU - Pols, H. A.P.

AU - van Leeuwen, J. P.T.M.

AU - Schilte, J. P.

AU - Visser, T. J.

AU - Birkenhäger, J. C.

PY - 1988/10/14

Y1 - 1988/10/14

N2 - 1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) receptor content in cultured osteogenic sarcoma cells (UMR-106) was found to be increased after treatment with both bovine and human PTH and human PTH-like peptide (hPLP). The dose dependent increase of receptors was preceded by a dose dependent stimulation of cAMP production. This suggests a role for cAMP as mediator of the PTH- and hPLP-induced 1,25-(OH)2D3 receptor up-regulation. Furthermore, evidence was obtained that new mRNA and de novo receptor synthesis is involved in this heterologous 1,25-(OH)2D3 receptor up-regulation.

AB - 1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) receptor content in cultured osteogenic sarcoma cells (UMR-106) was found to be increased after treatment with both bovine and human PTH and human PTH-like peptide (hPLP). The dose dependent increase of receptors was preceded by a dose dependent stimulation of cAMP production. This suggests a role for cAMP as mediator of the PTH- and hPLP-induced 1,25-(OH)2D3 receptor up-regulation. Furthermore, evidence was obtained that new mRNA and de novo receptor synthesis is involved in this heterologous 1,25-(OH)2D3 receptor up-regulation.

UR - https://www.scopus.com/pages/publications/0024286303

U2 - 10.1016/S0006-291X(88)80883-0

DO - 10.1016/S0006-291X(88)80883-0

M3 - Article

C2 - 2845985

AN - SCOPUS:0024286303

SN - 0006-291X

VL - 156

SP - 588

EP - 594

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Heterologous up-regulation of the 1,25-dihydroxyvitamin D₃ receptor by parathyroid hormone (PTH) and PTH-like peptide in osteoblast-like cells

Abstract

Access to Document

Other files and links

Fingerprint

Cite this