TY - JOUR
T1 - High Levels of Neutrophil Extracellular Traps Persist in the Lower Respiratory Tract of Critically Ill Patients with Coronavirus Disease 2019
AU - Ouwendijk, Werner J.D.
AU - Raadsen, Matthijs P.
AU - Van Kampen, Jeroen J.A.
AU - Verdijk, Robert M.
AU - Von Der Thusen, Jan H.
AU - Guo, Lihui
AU - Hoek, Rogier A.S.
AU - Van Den Akker, Johannes P.C.
AU - Endeman, Henrik
AU - Langerak, Thomas
AU - Molenkamp, Richard
AU - Gommers, Diederik
AU - Koopmans, Marion P.G.
AU - Van Gorp, Eric C.M.
AU - Verjans, Georges M.G.M.
AU - Haagmans, Bart L.
N1 - Publisher Copyright:
© 2021 The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].
PY - 2021/5/1
Y1 - 2021/5/1
N2 - Lower respiratory tract (LRT) disease induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can deteriorate to acute respiratory distress syndrome (ARDS). Because the release of neutrophil extracellular traps (NETs) is implicated in ARDS pathogenesis, we investigated the presence of NETs and correlates of pathogenesis in blood and LRT samples of critically ill patients with COVID-19. Plasma NET levels peaked early after intensive care unit admission and were correlated with the SARS-CoV-2 RNA load in sputum and levels of neutrophil-recruiting chemokines and inflammatory markers in plasma samples. The baseline plasma NET quantity was correlated with disease severity but was not associated with soluble markers of thrombosis or with development of thrombosis. High NET levels were present in LRT samples and persisted during the course of COVID-19, consistent with the detection of NETs in bronchi and alveolar spaces in lung tissue from deceased patient with COVID-19. Thus, NETs are produced and retained in the LRT of critically ill patients with COVID-19 and could contribute to SARS-CoV-2-induced ARDS disease.
AB - Lower respiratory tract (LRT) disease induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can deteriorate to acute respiratory distress syndrome (ARDS). Because the release of neutrophil extracellular traps (NETs) is implicated in ARDS pathogenesis, we investigated the presence of NETs and correlates of pathogenesis in blood and LRT samples of critically ill patients with COVID-19. Plasma NET levels peaked early after intensive care unit admission and were correlated with the SARS-CoV-2 RNA load in sputum and levels of neutrophil-recruiting chemokines and inflammatory markers in plasma samples. The baseline plasma NET quantity was correlated with disease severity but was not associated with soluble markers of thrombosis or with development of thrombosis. High NET levels were present in LRT samples and persisted during the course of COVID-19, consistent with the detection of NETs in bronchi and alveolar spaces in lung tissue from deceased patient with COVID-19. Thus, NETs are produced and retained in the LRT of critically ill patients with COVID-19 and could contribute to SARS-CoV-2-induced ARDS disease.
UR - http://www.scopus.com/inward/record.url?scp=85106861525&partnerID=8YFLogxK
U2 - 10.1093/infdis/jiab050
DO - 10.1093/infdis/jiab050
M3 - Article
C2 - 33507309
AN - SCOPUS:85106861525
SN - 0022-1899
VL - 223
SP - 1512
EP - 1521
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 9
ER -
