Host Factors Determine Anti-GM1 Response Following Oral Challenge of Chickens with Guillain-Barre Syndrome Derived Campylobacter jejuni Strain GB11

CW (Wim) Ang, JR Dijkstra, MA de Klerk, Hubert Endtz, Pieter van Doorn, B.C. Jacobs, SHM Jeurissen, JA Wagenaar

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Abstract

Background: Anti-ganglioside antibodies with a pathogenic potential are present in C. jejuni-associated Guillain-Barre syndrome (GBS) patients and are probably induced by molecular mimicry. Immunization studies in rabbits and mice have demonstrated that these anti-ganglioside antibodies can be induced using purified lipo-oligosaccharides (LOS) from C. jejuni in a strong adjuvant. Methodology/Principal Findings: To investigate whether natural colonization of chickens with a ganglioside-mimicking C. jejuni strain induces an anti-ganglioside response, and to investigate the diversity in anti-ganglioside response between and within genetically different chicken lines, we orally challenged chickens with different C. jejuni strains. Oral challenge of chickens with a C. jejuni strain from a GBS patient, containing a LOS that mimics ganglioside GM1, induced specific IgM and IgG anti-LOS and anti-GM1 antibodies. Inoculation of chickens with the Penner HS:3 serostrain, without a GM1-like structure, induced anti-LOS but no anti-ganglioside antibodies. We observed different patterns of anti-LOS/ganglioside response between and within the five strains of chickens. Conclusions: Natural infection of chickens with C. jejuni induces anti-ganglioside antibodies. The production of antibodies is governed by both microbial and host factors.
Original languageUndefined/Unknown
JournalPLoS One (print)
Volume5
Issue number3
DOIs
Publication statusPublished - 2010

Research programs

  • EMC MM-02-72-02
  • EMC MM-04-28-01
  • EMC MM-04-44-02

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