Human Endogenous Retrovirus K(HML-2) Gag- and Env-Specific T-Cell Responses Are Infrequently Detected in HIV-1-Infected Subjects Using Standard Peptide Matrix-Based Screening

RB Jones, VM John, DV Hunter, E Martin, S Mujib, V Mihajlovic, Peter Burgers, Theo Luider, G Gyenes, NC Sheppard, D SenGupta, R Tandon, FY Yue, E Benko, C Kovacs, DF Nixon, MA Ostrowski

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Abstract

T-cell responses to human endogenous retrovirus (HERV)K(HML-2) Gag and Env were mapped in HIV-1-infected subjects using 15mer peptides. Small peptide pools and high concentrations were used to maximize sensitivity. In the 23 subjects studied, only three bona fide HERV-K(HML-2)-specific responses were detected. At these high peptide concentrations, we detected false-positive responses, three of which were mapped to an HIV-1 Gag peptide contaminant. Thus, HERV-K(HML-2) Gag-and Env-specific T-cell responses are infrequently detected by 15mer peptide mapping.
Original languageUndefined/Unknown
Pages (from-to)288-292
Number of pages5
JournalClinical and Vaccine Immunology
Volume19
Issue number2
DOIs
Publication statusPublished - 2012

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