Human ovarian tumors of epithelial origin express PDGF in vitro and in vivo

Marjan A. Versnel*, Melang Haarbrink, Anthonie W. Langerak, Petronella A.J.M. de Laat, Anne Hagemeijer, Theo H. van der Kwast, Lia A.M. van den Berg-Bakker, Peter I. Schrier

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

39 Citations (Scopus)

Abstract

In human malignant mesothelioma cell lines an elevation of the expression of the genes for the PDGF A-chain, PDGF B-chain, and PDGF β-receptor was found compared to normal mesothelial cells. As ovarian epithelial tumors originate from the ovarian surface epithelium, which is of mesothelial origin, we investigated PDGF chain and PDGF receptor mRNA expression in six human ovarian cell lines of epithelial origin and a granulosa tumor cell line. All six investigated ovarian epithelial tumor cell lines expressed the PDGF A- and B-chain genes, while the granulosa tumor cell line expressed the PDGF A-chain gene only. Expression of PDGF receptors was not found in the epithelial or granulosa tumor cell lines. Cytogenetic and molecular biological studies did not provide evidence for rearrangement or genomic amplification of the PDGF B-chain. Expression of PDGF was also demonstrated in ovarian tumors in vivo. Frozen sections of six serous ovarian carcinomas stained positive with an antibody against PDGF and negative with antibodies against the PDGF α- and β-receptors. These results suggest that PDGF expression might be a useful marker for ovarian carcinomas.

Original languageEnglish
Pages (from-to)60-64
Number of pages5
JournalCancer Genetics and Cytogenetics
Volume73
Issue number1
DOIs
Publication statusPublished - Mar 1994

Bibliographical note

We are grateful to Professor Dr. R. Benner for his continuous support and to Mrs. M.J. Bouts, Ms. E. Postma, Dr. S. Chadha-Ajwani, and Dr. M.E. Vierhout for their advice and technical assistance. Dr. L. Claesson-Walsh kindly provided the probes for the PDGF a-and ~-receptor. Mr. T.M. van Os is acknowledged for excallent photographic assistance and Mr. H.J. Elsenbreek-de Jager for typing the manuscript.

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