Hydrocortisone-induced increase of PDGF β-receptor expression in a human malignant mesothelioma cell line

M. A. Versnel*, M. J. Bouts, A. W. Langerak, Th H. vander Kwast, H. C. Hoogsteden, A. Hagemeijer, C. H. Heldin

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

The effect of hydrocortisone (HC) on PDGF β-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF β-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF β-receptor gene. The expression of PDGF β-receptor protein was also elevated by HC as demonstrated with an immunoblotting assay. However, the number of PDGF-BB binding sites on the cell surface of Mero-14 remained unchanged upon HC treatment. These results suggest that steroid hormones can regulate PDGF receptor expression in vivo.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalExperimental Cell Research
Volume200
Issue number1
DOIs
Publication statusPublished - May 1992

Bibliographical note

We are grateful to Prof. Dr. R. Benner for his continuous support and Ms. E. Postma and Mr. M. Delahaye for their advice and technical assistance. Ms. H. Vietsch is acknowledged for the immunoblotting experiments and Mr. T. M. van Os for excellent photographic assistance. Dr. L. Claesson-Welsh is acknowledged for generously providing the PDGF a-and ~-receptor probes and Mrs. H. J. Elsen-broek-de Jager for typing the manuscript.

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