Hydrocortisone-induced increase of PDGF β-receptor expression in a human malignant mesothelioma cell line

M. A. Versnel; M. J. Bouts; A. W. Langerak; Th H. vander Kwast; H. C. Hoogsteden; A. Hagemeijer; C. H. Heldin

doi:10.1016/S0014-4827(05)80074-6

Hydrocortisone-induced increase of PDGF β-receptor expression in a human malignant mesothelioma cell line

M. A. Versnel^*
, M. J. Bouts
, A. W. Langerak
, Th H. vander Kwast
, H. C. Hoogsteden
, A. Hagemeijer
, C. H. Heldin

^*Corresponding author for this work

University Hospital Dijkzigt
Ludwig Institute for Cancer Research

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

The effect of hydrocortisone (HC) on PDGF β-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF β-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF β-receptor gene. The expression of PDGF β-receptor protein was also elevated by HC as demonstrated with an immunoblotting assay. However, the number of PDGF-BB binding sites on the cell surface of Mero-14 remained unchanged upon HC treatment. These results suggest that steroid hormones can regulate PDGF receptor expression in vivo.

Original language	English
Pages (from-to)	83-88
Number of pages	6
Journal	Experimental Cell Research
Volume	200
Issue number	1
DOIs	https://doi.org/10.1016/S0014-4827(05)80074-6
Publication status	Published - May 1992

Bibliographical note

We are grateful to Prof. Dr. R. Benner for his continuous support and Ms. E. Postma and Mr. M. Delahaye for their advice and technical assistance. Ms. H. Vietsch is acknowledged for the immunoblotting experiments and Mr. T. M. van Os for excellent photographic assistance. Dr. L. Claesson-Welsh is acknowledged for generously providing the PDGF a-and ~-receptor probes and Mrs. H. J. Elsen-broek-de Jager for typing the manuscript.

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@article{d6cdcb1bb6ea47898fa6a86c5eac0f9b,

title = "Hydrocortisone-induced increase of PDGF β-receptor expression in a human malignant mesothelioma cell line",

abstract = "The effect of hydrocortisone (HC) on PDGF β-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF β-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF β-receptor gene. The expression of PDGF β-receptor protein was also elevated by HC as demonstrated with an immunoblotting assay. However, the number of PDGF-BB binding sites on the cell surface of Mero-14 remained unchanged upon HC treatment. These results suggest that steroid hormones can regulate PDGF receptor expression in vivo.",

author = "Versnel, \{M. A.\} and Bouts, \{M. J.\} and Langerak, \{A. W.\} and \{vander Kwast\}, \{Th H.\} and Hoogsteden, \{H. C.\} and A. Hagemeijer and Heldin, \{C. H.\}",

note = "We are grateful to Prof. Dr. R. Benner for his continuous support and Ms. E. Postma and Mr. M. Delahaye for their advice and technical assistance. Ms. H. Vietsch is acknowledged for the immunoblotting experiments and Mr. T. M. van Os for excellent photographic assistance. Dr. L. Claesson-Welsh is acknowledged for generously providing the PDGF a-and \textasciitilde{}-receptor probes and Mrs. H. J. Elsen-broek-de Jager for typing the manuscript. ",

year = "1992",

month = may,

doi = "10.1016/S0014-4827(05)80074-6",

language = "English",

volume = "200",

pages = "83--88",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Elsevier Inc.",

number = "1",

}

TY - JOUR

T1 - Hydrocortisone-induced increase of PDGF β-receptor expression in a human malignant mesothelioma cell line

AU - Versnel, M. A.

AU - Bouts, M. J.

AU - Langerak, A. W.

AU - vander Kwast, Th H.

AU - Hoogsteden, H. C.

AU - Hagemeijer, A.

AU - Heldin, C. H.

N1 - We are grateful to Prof. Dr. R. Benner for his continuous support and Ms. E. Postma and Mr. M. Delahaye for their advice and technical assistance. Ms. H. Vietsch is acknowledged for the immunoblotting experiments and Mr. T. M. van Os for excellent photographic assistance. Dr. L. Claesson-Welsh is acknowledged for generously providing the PDGF a-and ~-receptor probes and Mrs. H. J. Elsen-broek-de Jager for typing the manuscript.

PY - 1992/5

Y1 - 1992/5

N2 - The effect of hydrocortisone (HC) on PDGF β-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF β-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF β-receptor gene. The expression of PDGF β-receptor protein was also elevated by HC as demonstrated with an immunoblotting assay. However, the number of PDGF-BB binding sites on the cell surface of Mero-14 remained unchanged upon HC treatment. These results suggest that steroid hormones can regulate PDGF receptor expression in vivo.

AB - The effect of hydrocortisone (HC) on PDGF β-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF β-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF β-receptor gene. The expression of PDGF β-receptor protein was also elevated by HC as demonstrated with an immunoblotting assay. However, the number of PDGF-BB binding sites on the cell surface of Mero-14 remained unchanged upon HC treatment. These results suggest that steroid hormones can regulate PDGF receptor expression in vivo.

UR - https://www.scopus.com/pages/publications/0026695033

U2 - 10.1016/S0014-4827(05)80074-6

DO - 10.1016/S0014-4827(05)80074-6

M3 - Article

C2 - 1314190

AN - SCOPUS:0026695033

SN - 0014-4827

VL - 200

SP - 83

EP - 88

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 1

ER -

Hydrocortisone-induced increase of PDGF β-receptor expression in a human malignant mesothelioma cell line

Abstract

Bibliographical note

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