Identification and typing of isolates of Cyphellophora and relatives by use of amplified fragment length polymorphism and rolling circle amplification

Peiying Feng, Corné H W Klaassen, Jacques F Meis, M J Najafzadeh, A H G Gerrits Van Den Ende, Liyan Xi, G S de Hoog*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)

Abstract

The species diversity and identification of black fungi belonging to Cyphellophora and Phialophora, which colonize and infect human skin and nails, were studied using amplified fragment length polymorphism (AFLP). A total of 76 Cyphellophora and Phialophora isolates were evaluated, and their delimitation was compared to earlier studies using multilocus sequencing. The results of the AFLP analysis and sequencing were in complete agreement with each other. Seven species-specific padlock probes for the most prevalent species were designed on the basis of the ribosomal DNA internal transcribed spacer region, and identification of the respective species could easily be achieved with the aid of rolling circle amplification.

Original languageEnglish
Pages (from-to)931-937
Number of pages7
JournalJournal of Clinical Microbiology
Volume51
Issue number3
DOIs
Publication statusPublished - Mar 2013
Externally publishedYes

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