TY - JOUR
T1 - Identification of a Spike-Specific CD8+ T-Cell Epitope Following Vaccination Against the Middle East Respiratory Syndrome Coronavirus in Humans
AU - Harrer, Caroline E.
AU - Mayer, Leonie
AU - MVA-MERS-S Study Group
AU - Fathi, Anahita
AU - Lassen, Susan
AU - Ly, My L.
AU - Zinser, Madeleine E.
AU - Wolf, Timo
AU - Becker, Stephan
AU - Sutter, Gerd
AU - Dahlke, Christine
AU - Addo, Marylyn M.
AU - Bartels, Etienne
AU - Friedrich, Monika
AU - Weskamm, Leonie M.
AU - Grundlach, Swantje
AU - Poetsch, Joseph H.
AU - Koch, Till
AU - Schmiedel, Stefan
AU - Haagmanns, Bart
AU - Hesterkamp, Thomas
AU - Krähling, Verena
AU - Volz, Asisa
AU - Ditt, Vanessa A.
AU - Kessler, Melanie
N1 - Publisher Copyright:
© 2024 The Author(s).
PY - 2024/8/15
Y1 - 2024/8/15
N2 - Licensed vaccines against the Middle East respiratory syndrome coronavirus (MERS-CoV), an emerging pathogen of concern, are lacking. The modified vaccinia virus Ankara vector-based vaccine MVA-MERS-S, expressing the MERS-CoV-spike glycoprotein (MERS-S), is one of 3 candidate vaccines in clinical development and elicits robust humoral and cellular immunity. Here, we identified for the first time a MERS-S-specific CD8+ T-cell epitope in an HLA-A∗03:01/HLA-B∗35:01-positive vaccinee using a screening assay, intracellular cytokine staining, and in silico epitope prediction. As evidence from MERS-CoV infection suggests a protective role of long-lasting CD8+ T-cell responses, the identification of epitopes will facilitate longitudinal analyses of vaccine-induced T-cell immunity.
AB - Licensed vaccines against the Middle East respiratory syndrome coronavirus (MERS-CoV), an emerging pathogen of concern, are lacking. The modified vaccinia virus Ankara vector-based vaccine MVA-MERS-S, expressing the MERS-CoV-spike glycoprotein (MERS-S), is one of 3 candidate vaccines in clinical development and elicits robust humoral and cellular immunity. Here, we identified for the first time a MERS-S-specific CD8+ T-cell epitope in an HLA-A∗03:01/HLA-B∗35:01-positive vaccinee using a screening assay, intracellular cytokine staining, and in silico epitope prediction. As evidence from MERS-CoV infection suggests a protective role of long-lasting CD8+ T-cell responses, the identification of epitopes will facilitate longitudinal analyses of vaccine-induced T-cell immunity.
UR - http://www.scopus.com/inward/record.url?scp=85201437577&partnerID=8YFLogxK
U2 - 10.1093/infdis/jiad612
DO - 10.1093/infdis/jiad612
M3 - Article
C2 - 38195212
AN - SCOPUS:85201437577
SN - 0022-1899
VL - 230
SP - e327-e332
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -