Identification of microRNAs in human plasma

Bram C. Van Der Eerden*, Rodrigo D. Alves, Christel E. Kockx, Zeliha Ozgur, Marijke Schreuders-Koedam, Jeroen Van De Peppel, Wilfred F. Van Ijcken, Johannes P. Van Leeuwen

*Corresponding author for this work

Research output: Chapter/Conference proceedingChapterAcademic

1 Citation (Scopus)


In recent years, microRNAs (miRNA) have been demonstrated to be present in body fluids and may therefore serve as diagnostic markers for diseases. By characterizing miRNA profiles in plasma, a miRNA signature may potentially be developed as a diagnostic and risk assessment tool for particular (patho)physiological states. This chapter describes the isolation, purification, identification, and sequencing of human plasma miRNAs.

Original languageEnglish
Title of host publicationOsteoporosis and Osteoarthritis
EditorsJennifer J. Westendorf, Andre J. van Wijnen
Number of pages15
ISBN (Electronic)9781493916191
Publication statusPublished - 20 Oct 2014

Publication series

SeriesMethods in Molecular Biology

Bibliographical note

Publisher Copyright: © Springer Science+Business Media New York 2015. All rights are reserved.

This work was supported by the Netherlands Organization for Scientific Research (NWO; NGI Horizon grant 93511016)

Research programs

  • EMC MGC-02-13-02
  • EMC MM-01-39-02


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