Identification of multiple mRNA and DNA sequences from small tissue samples isolated by laser-assisted microdissection

Monique R. Bernsen*, Henry B.P.M. Dijkman, Edwin De Vries, Carl G. Figdor, Dirk J. Ruiter, Gosse J. Adema, Goos N.P. Van Muijen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

55 Citations (Scopus)

Abstract

Molecular analysis of small tissue samples has become increasingly important in biomedical studies. Using a laser dissection microscope and modified nucleic acid isolation protocols, we demonstrate that multiple mRNA as well as DNA sequences can be identified from a single-cell sample. In addition, we show that the specificity of procurement of tissue samples is not compromised by smear contamination resulting from scraping of the microtome knife during sectioning of lesions. The procedures described herein thus allow for efficient RT-PCR or PCR analysis of multiple nucleic acid sequences from small tissue samples obtained by laser-assisted microdissection.

Original languageEnglish
Pages (from-to)1267-1273
Number of pages7
JournalLaboratory Investigation
Volume78
Issue number10
Publication statusPublished - Oct 1998
Externally publishedYes

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