In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons

Robin Broersen; Greg J. Stuart

doi:10.1016/j.xpro.2022.101963

In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons

Robin Broersen^*, Greg J. Stuart

^*Corresponding author for this work

Neurosciences

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Abstract

In vivo whole-cell recording, when combined with morphological characterization after biocytin labeling, is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we describe steps for performing whole-cell recordings in the superior colliculus of urethane-anesthetized mice, a major visual processing region in the rodent brain. We detail two types of visual stimulation techniques: full-field light-emitting diode (LED) flashes and visual stimuli shown on monitors. While we focus on superior colliculus, this protocol is applicable to other brain areas.

Original language	English
Article number	101963
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.101963
Publication status	Published - 17 Mar 2023

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10.1016/j.xpro.2022.101963Licence: CC BY-NC-ND

1-s2.0-S2666166722008437-mainFinal published version, 4.01 MBLicence: CC BY-NC-ND

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In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons

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