IL-1 differently stimulates proliferation and multinucleation of distinct mouse bone marrow osteoclast precursor subsets

YX Cao, IDC Jansen, S Sprangers, J Stap, Pieter Leenen, V Everts, TJ de Vries

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IL-1 has diverse stimulatory effects on different bone marrow osteoclast precursors. Osteoclasts are bone-resorbing cells and targets for treating bone diseases. Previously, we reported that distinct murine osteoclast precursor subsets, such as early blasts (CD31(hi) Ly-6C(-)), myeloid blasts (CD31(+) Ly-6C(+)), and monocytes (CD31(-) Ly-6C(hi)), respond differently to the osteoclastogenesis-inducing cytokines, macrophage colony-stimulating factor, and receptor activator for nuclear factor B ligand. It is unknown, however, how these cell types respond to the osteoclast-stimulating inflammatory cytokine interleukin 1. This study aims to investigate the effect of interleukin 1 on osteoclastogenesis derived from different mouse bone marrow precursors. Early blasts, myeloid blasts, and monocytes were sorted from mouse bone marrow cells using flow cytometry. Cells were cultured on plastic or on bone slices in the presence of macrophage colony-stimulating factor and receptor activator for nuclear factor B ligand, without or with interleukin 1 (0.1-10 ng/ml). We found that interleukin 1 stimulated multinucleation and bone resorption of osteoclasts derived from the 3 precursors at different rates. The most large osteoclasts (>20 nuclei) and highest level of bone resorption (16.3%) was by myeloid blast-derived osteoclasts. Interleukin 1 particularly accelerated proliferation of early blasts and the most small osteoclasts (3-5 nuclei) formed on plastic. Life span varied among osteoclasts derived from different precursors: large osteoclasts (>2400 mu m(2)) formed most rapidly (75 h) from myeloid blasts but had a short life span (30 h). Monocytes needed the longest time (95 h) for the generation of such large osteoclasts, but these cells had a longer life span (50 h). Our results indicate that the different bone marrow osteoclast precursors are differently stimulated by interleukin 1 with respect to proliferation, multinucleation, life span, and bone resorption.
Original languageUndefined/Unknown
Pages (from-to)513-523
Number of pages11
JournalJournal of Leukocyte Biology
Issue number3
Publication statusPublished - 2016

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