TY - JOUR
T1 - IL-10-Secreting Regulatory T Cells Do Not Express Foxp3 but Have Comparable Regulatory Function to Naturally Occurring CD4+CD25 + Regulatory T Cells
AU - Vieira, Pedro L.
AU - Christensen, Jillian R.
AU - Minaee, Sophie
AU - O'Neil, Emma J.
AU - Barrat, Franck J.
AU - Boonstra, André
AU - Barthlott, Thomas
AU - Stockinger, Brigitta
AU - Wraith, David C.
AU - O'Garra, Anne
PY - 2004/5/15
Y1 - 2004/5/15
N2 - Regulatory T cells (TReg) control immune responses to self and nonself Ags. The relationship between Ag-driven IL-10-secreting TReg (EL-10-TReg) and naturally occurring CD4+CD25+ TReg is as yet unclear. We show that mouse IL-10-TReg obtained using either in vitro or in vivo regimens of antigenic stimulation did not express the CD4+CD25+ TReg-associated transcription factor Foxp3. However, despite the absence of Foxp3 expression, homogeneous populations of IL-10-TReg inhibited the in vitro proliferation of CD4+CD25- T cells with a similar efficiency to that of CD4+CD25+ TReg. This inhibition of T cell proliferation by IL-10-TReg was achieved through an IL-10-independent mechanism as seen for CD4+CD25 + TReg and was overcome by exogenous IL-2. Both IL-10-TReg and CD4+CD25+ TReg were similar in that they produced little to no IL-2. These data show that Foxp3 expression is not a prerequisite for IL-10-TReg activity in vitro or in vivo, and suggest that IL-10-TReg and naturally occurring CD4 +CD25+ TReg may have distinct origins.
AB - Regulatory T cells (TReg) control immune responses to self and nonself Ags. The relationship between Ag-driven IL-10-secreting TReg (EL-10-TReg) and naturally occurring CD4+CD25+ TReg is as yet unclear. We show that mouse IL-10-TReg obtained using either in vitro or in vivo regimens of antigenic stimulation did not express the CD4+CD25+ TReg-associated transcription factor Foxp3. However, despite the absence of Foxp3 expression, homogeneous populations of IL-10-TReg inhibited the in vitro proliferation of CD4+CD25- T cells with a similar efficiency to that of CD4+CD25+ TReg. This inhibition of T cell proliferation by IL-10-TReg was achieved through an IL-10-independent mechanism as seen for CD4+CD25 + TReg and was overcome by exogenous IL-2. Both IL-10-TReg and CD4+CD25+ TReg were similar in that they produced little to no IL-2. These data show that Foxp3 expression is not a prerequisite for IL-10-TReg activity in vitro or in vivo, and suggest that IL-10-TReg and naturally occurring CD4 +CD25+ TReg may have distinct origins.
UR - http://www.scopus.com/inward/record.url?scp=2442450433&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.172.10.5986
DO - 10.4049/jimmunol.172.10.5986
M3 - Article
C2 - 15128781
AN - SCOPUS:2442450433
SN - 0022-1767
VL - 172
SP - 5986
EP - 5993
JO - Journal of Immunology
JF - Journal of Immunology
IS - 10
ER -