IL-17 produced by Paneth cells drives TNF-induced shock

N Takahashi, I Vanlaere, R de Rycke, A Cauwels, LAB Joosten, Erik Lubberts, WB van den Berg, C Libert

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Abstract

Tumor necrosis factor (TNF) has very potent antitumor activity, but it also provokes a systemic inflammatory response syndrome that leads to shock, organ failure, and death. Here, we demonstrate that interleukin (IL)-17, a proinflammatory cytokine known to be produced mainly by activated T cells, has a critical role in this process. Antiserum against IL-17 or deletion of Il17r protected mice against a lethal TNF challenge. Serum levels of TNF-induced IL-6 and nitric oxide metabolites were significantly reduced in mice deficient in the IL-17R. TNF-induced leukocyte influx in the small intestine was reduced, and there was no injury to the small intestine. Surprisingly, electron microscopy showed that IL-17 was constitutively present in Paneth cells of the crypts. Upon TNF challenge, the intracellular pool of IL-17 in these cells was drastically reduced, suggesting rapid release of IL-17 from the granules of Paneth cells. Our findings assign a novel role for IL-17 in an acute inflammation and identify Paneth cells as a source of the IL-17 that plays a role in this process. These data indicate that innate immune cytokine responses in the local mucosa may participate in rapidly amplifying responses to systemic inflammatory challenges.
Original languageUndefined/Unknown
Pages (from-to)1755-1761
Number of pages7
JournalJournal of Experimental Medicine
Volume205
Issue number8
DOIs
Publication statusPublished - 2008

Research programs

  • EMC MUSC-01-31-01

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