TY - JOUR
T1 - Imaging mass cytometry reveals the prominent role of myeloid cells at the maternal-fetal interface
AU - Krop, Juliette
AU - van der Zwan, Anita
AU - Ijsselsteijn, Marieke E
AU - Kapsenberg, Hanneke
AU - Luk, Sietse J
AU - Hendriks, Sanne H
AU - van der Keur, Carin
AU - Verleng, Lotte J
AU - Somarakis, Antonis
AU - van der Meeren, Lotte
AU - Haasnoot, Geert
AU - Bos, Manon
AU - de Miranda, Noel F C C
AU - Chuva de Sousa Lopes, Susana M
AU - van der Hoorn, Marie-Louise P
AU - Koning, Frits
AU - Claas, Frans H J
AU - Heidt, Sebastiaan
AU - Eikmans, Michael
N1 - Publisher Copyright:
© 2022 The Authors
PY - 2022/7/15
Y1 - 2022/7/15
N2 - Although the immunological complexity of the maternal-fetal interface is well appreciated, the actual interaction of maternal immune cells and fetal trophoblasts is insufficiently understood. To comprehend the composition and spatial orientation of maternal immune cells and fetal extravillous trophoblasts, we applied imaging mass cytometry on decidua basalis of the three trimesters of healthy pregnancy. Within all trimesters, we observed considerably higher frequencies of myeloid cells in the decidua than is seen with single-cell suspension techniques. Moreover, they were the most pronounced cell type in the microenvironment of other decidual cells. In first trimester, HLA-DR- macrophages represented the most abundant myeloid subcluster and these cells were frequently observed in the vicinity of trophoblasts. At term, HLA-DR+ macrophage subclusters were abundantly present and frequently observed in the microenvironment of T cells. Taken together, our results highlight the dynamic role of myeloid cells at the human maternal-fetal interface throughout gestation.
AB - Although the immunological complexity of the maternal-fetal interface is well appreciated, the actual interaction of maternal immune cells and fetal trophoblasts is insufficiently understood. To comprehend the composition and spatial orientation of maternal immune cells and fetal extravillous trophoblasts, we applied imaging mass cytometry on decidua basalis of the three trimesters of healthy pregnancy. Within all trimesters, we observed considerably higher frequencies of myeloid cells in the decidua than is seen with single-cell suspension techniques. Moreover, they were the most pronounced cell type in the microenvironment of other decidual cells. In first trimester, HLA-DR- macrophages represented the most abundant myeloid subcluster and these cells were frequently observed in the vicinity of trophoblasts. At term, HLA-DR+ macrophage subclusters were abundantly present and frequently observed in the microenvironment of T cells. Taken together, our results highlight the dynamic role of myeloid cells at the human maternal-fetal interface throughout gestation.
UR - http://www.scopus.com/inward/record.url?scp=85133246749&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2022.104648
DO - 10.1016/j.isci.2022.104648
M3 - Article
C2 - 35811852
VL - 25
JO - iScience
JF - iScience
IS - 7
M1 - 104648
ER -
