Imaging of experimental osteoarthritis in small animal models

Tom Piscaer, Gerjo van Osch, Jan Verhaar, HH Weinans

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)


Normally, tissue alterations in small animal models for osteoarthritis (OA) are assessed by time-consuming and destructive histology or biochemical assays. Some high resolution imaging modalities are used for longitudinal monitoring of the OA disease process in vivo. mu CT is one of these imaging modalities, which is known for superb high-resolution imaging of bone architecture alterations. A major drawback of mu CT is that it has low soft-tissue contrast, which makes direct imaging of cartilage impossible. The use of mu CT in combination with negatively charged radiopaque contrast agents enables imaging of cartilage degeneration. We demonstrate the possibility of mu CT to image cartilage degeneration as a consequence of experimental OA, by the use contrast enhanced mu CT in vivo in a rat model for OA. Furthermore, for the assessment of alterations in molecular processes involved in OA we used the recently developed technique of multi pinhole SPECT. This enables us to assess molecular processes involved in experimental OA in a rat at sub-millimeter level. Here we show quantification of subchondral bone turnover in an OA rat knee. These new techniques demonstrate the possibilities of quantitative experimental OA assessment in small animal models such as mice and rats and might enable substitution of the conventional destructive methods.
Original languageUndefined/Unknown
Pages (from-to)355-364
Number of pages10
Issue number3-4
Publication statusPublished - 2008

Research programs

  • EMC MUSC-01-51-01
  • EMC OR-01-62-02

Cite this