Immuno-effector characteristics of peritoneal cells during CAPD treatment: a longitudinal study

M G Betjes; C W Tuk; D G Struijk; R T Krediet; L Arisz; E C Hoefsmit; R H Beelen

doi:10.1038/ki.1993.93

Immuno-effector characteristics of peritoneal cells during CAPD treatment: a longitudinal study

M G Betjes, C W Tuk, D G Struijk, R T Krediet, L Arisz, E C Hoefsmit, R H Beelen

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Abstract

Peritoneal cells (PC) from 75 patients were immuno-phenotypically and functionally characterized during the first year of CAPD treatment (PCcapd) and compared to PC obtained by laparoscopy of healthy women (control peritoneal cells). Patients were divided, according to their peritonitis incidence (PI), into a high PI (HPI) and a low PI group (LPI). The yield of PCcapd decreased significantly over the year. The differential cell count and immunophenotype of PCcapd remained unchanged in the LPI group, but the percentage of macrophages decreased over the year in the HPI group. Macrophages in the PCcapd, when compared to control peritoneal cells, had a less mature phenotype as measured by RFD7 expression but a higher Fc-receptor expression. The PCcapd showed a higher percentage of B cells, CD4 positive T cells and activated T cells bearing HLA-DR/DQ when compared to the control peritoneal cells. Over the year a decrease in chemotactic activity of the PCcapd towards 10(-8) M N-formylmethionyl-leucyl-phenylalanine and dialysis effluent was observed in LPI patients but not in HPI patients. After one year of treatment, a significantly higher percentage of phagocytosing macrophages in the PCcapd of HPI patients was found when compared to LPI patients. During the year there was an increase of immunophagocytosis of PCcapd independent of PI. In conclusion, the CAPD peritoneal cellular immune system showed signs of both immaturity and activation. The decrease in the yield and in the chemotactic activity of PCcapd suggests an adaptation to the chronic stimulus of the dialysis fluid.(ABSTRACT TRUNCATED AT 250 WORDS)

Original language	English
Pages (from-to)	641-8
Number of pages	8
Journal	Kidney International
Volume	43
Issue number	3
DOIs	https://doi.org/10.1038/ki.1993.93
Publication status	Published - Mar 1993
Externally published	Yes

Bibliographical note

Acknowledgments
This study was supported by grant D89.866 of the Dutch KidneyFoundation (Nier Stichting Nederland, Bussum). We are grateful to
Dr. C.W. Burger for providing the laparoscopy samples. We also wish to thank Mr. J. van der Ploeg for his support.

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title = "Immuno-effector characteristics of peritoneal cells during CAPD treatment: a longitudinal study",

abstract = "Peritoneal cells (PC) from 75 patients were immuno-phenotypically and functionally characterized during the first year of CAPD treatment (PCcapd) and compared to PC obtained by laparoscopy of healthy women (control peritoneal cells). Patients were divided, according to their peritonitis incidence (PI), into a high PI (HPI) and a low PI group (LPI). The yield of PCcapd decreased significantly over the year. The differential cell count and immunophenotype of PCcapd remained unchanged in the LPI group, but the percentage of macrophages decreased over the year in the HPI group. Macrophages in the PCcapd, when compared to control peritoneal cells, had a less mature phenotype as measured by RFD7 expression but a higher Fc-receptor expression. The PCcapd showed a higher percentage of B cells, CD4 positive T cells and activated T cells bearing HLA-DR/DQ when compared to the control peritoneal cells. Over the year a decrease in chemotactic activity of the PCcapd towards 10(-8) M N-formylmethionyl-leucyl-phenylalanine and dialysis effluent was observed in LPI patients but not in HPI patients. After one year of treatment, a significantly higher percentage of phagocytosing macrophages in the PCcapd of HPI patients was found when compared to LPI patients. During the year there was an increase of immunophagocytosis of PCcapd independent of PI. In conclusion, the CAPD peritoneal cellular immune system showed signs of both immaturity and activation. The decrease in the yield and in the chemotactic activity of PCcapd suggests an adaptation to the chronic stimulus of the dialysis fluid.(ABSTRACT TRUNCATED AT 250 WORDS)",

author = "Betjes, {M G} and Tuk, {C W} and Struijk, {D G} and Krediet, {R T} and L Arisz and Hoefsmit, {E C} and Beelen, {R H}",

note = "Acknowledgments This study was supported by grant D89.866 of the Dutch KidneyFoundation (Nier Stichting Nederland, Bussum). We are grateful to Dr. C.W. Burger for providing the laparoscopy samples. We also wish to thank Mr. J. van der Ploeg for his support.",

year = "1993",

month = mar,

doi = "10.1038/ki.1993.93",

language = "English",

volume = "43",

pages = "641--8",

journal = "Kidney International",

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T1 - Immuno-effector characteristics of peritoneal cells during CAPD treatment

T2 - a longitudinal study

AU - Betjes, M G

AU - Tuk, C W

AU - Struijk, D G

AU - Krediet, R T

AU - Arisz, L

AU - Hoefsmit, E C

AU - Beelen, R H

N1 - Acknowledgments This study was supported by grant D89.866 of the Dutch KidneyFoundation (Nier Stichting Nederland, Bussum). We are grateful to Dr. C.W. Burger for providing the laparoscopy samples. We also wish to thank Mr. J. van der Ploeg for his support.

PY - 1993/3

Y1 - 1993/3

N2 - Peritoneal cells (PC) from 75 patients were immuno-phenotypically and functionally characterized during the first year of CAPD treatment (PCcapd) and compared to PC obtained by laparoscopy of healthy women (control peritoneal cells). Patients were divided, according to their peritonitis incidence (PI), into a high PI (HPI) and a low PI group (LPI). The yield of PCcapd decreased significantly over the year. The differential cell count and immunophenotype of PCcapd remained unchanged in the LPI group, but the percentage of macrophages decreased over the year in the HPI group. Macrophages in the PCcapd, when compared to control peritoneal cells, had a less mature phenotype as measured by RFD7 expression but a higher Fc-receptor expression. The PCcapd showed a higher percentage of B cells, CD4 positive T cells and activated T cells bearing HLA-DR/DQ when compared to the control peritoneal cells. Over the year a decrease in chemotactic activity of the PCcapd towards 10(-8) M N-formylmethionyl-leucyl-phenylalanine and dialysis effluent was observed in LPI patients but not in HPI patients. After one year of treatment, a significantly higher percentage of phagocytosing macrophages in the PCcapd of HPI patients was found when compared to LPI patients. During the year there was an increase of immunophagocytosis of PCcapd independent of PI. In conclusion, the CAPD peritoneal cellular immune system showed signs of both immaturity and activation. The decrease in the yield and in the chemotactic activity of PCcapd suggests an adaptation to the chronic stimulus of the dialysis fluid.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - Peritoneal cells (PC) from 75 patients were immuno-phenotypically and functionally characterized during the first year of CAPD treatment (PCcapd) and compared to PC obtained by laparoscopy of healthy women (control peritoneal cells). Patients were divided, according to their peritonitis incidence (PI), into a high PI (HPI) and a low PI group (LPI). The yield of PCcapd decreased significantly over the year. The differential cell count and immunophenotype of PCcapd remained unchanged in the LPI group, but the percentage of macrophages decreased over the year in the HPI group. Macrophages in the PCcapd, when compared to control peritoneal cells, had a less mature phenotype as measured by RFD7 expression but a higher Fc-receptor expression. The PCcapd showed a higher percentage of B cells, CD4 positive T cells and activated T cells bearing HLA-DR/DQ when compared to the control peritoneal cells. Over the year a decrease in chemotactic activity of the PCcapd towards 10(-8) M N-formylmethionyl-leucyl-phenylalanine and dialysis effluent was observed in LPI patients but not in HPI patients. After one year of treatment, a significantly higher percentage of phagocytosing macrophages in the PCcapd of HPI patients was found when compared to LPI patients. During the year there was an increase of immunophagocytosis of PCcapd independent of PI. In conclusion, the CAPD peritoneal cellular immune system showed signs of both immaturity and activation. The decrease in the yield and in the chemotactic activity of PCcapd suggests an adaptation to the chronic stimulus of the dialysis fluid.(ABSTRACT TRUNCATED AT 250 WORDS)

U2 - 10.1038/ki.1993.93

DO - 10.1038/ki.1993.93

M3 - Article

C2 - 8455363

SN - 0085-2538

VL - 43

SP - 641

EP - 648

JO - Kidney International

JF - Kidney International

IS - 3

ER -

Immuno-effector characteristics of peritoneal cells during CAPD treatment: a longitudinal study

Abstract

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