Immunoglobulin and t-cell receptor gene analysis in the diagnosis of lymphoid malignancies

Anton W. Langerak*, Ingrid Wolvers-Tettero, Jacques L.M. van Dongen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)


Southern blotting and PCR analysis allow a detailed evaluation of the immunoglobulin (Ig) and Tcell receptor (TCFt) gene configuration in lymphoproliferations based on the combinatorial and junctional diversity of the gene .rearrangements, respectively. If appropriate probe/restriction enzyme combinations and appropriate primer sets are employed, both techniques can be used for diagnostic clonality studies. PCR-based analysis of Ig and TCP genes can only be performed in a reliable way if amplification is followed by PCR product analysis to discern between polyclonality and monoclonality. In the vast majority of suspect lymphoproliferations the IgH and TCRβ genes can be analyzed for clonality assessment via Southern blotting. If inconclusive results are obtained, the Igte and TCRygene complexes can be studied in a second step. PCR analyses generally focus on junctional regions of rearranged IgH and TCRy genes, as these are rearranged in the majority of B- and T-cell malignancies, respectively, and require only a limited number of primer sets. The possibilities and limitations of the application of both techniques are discussed in this review.

Original languageEnglish
Pages (from-to)3-27
Number of pages25
JournalReviews in Clinical and Experimental Hematology
Publication statusPublished - 1997


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