Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq

V. H. J. van der Velden*, L. Bastian, M. Brüggemann, A. M. Hartmann, N. Darzentas

*Corresponding author for this work

Research output: Chapter/Conference proceedingChapterAcademic

1 Citation (Scopus)
10 Downloads (Pure)

Abstract

Identification of immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements in acute lymphoblastic leukemia (ALL) patients at initial presentation are crucial for monitoring of minimal residual disease (MRD) during subsequent follow-up and thereby for appropriate risk-group stratification. Here we describe how RNA-Seq data can be generated and subsequently analyzed with ARResT/Interrogate to identify possible MRD markers. In addition to the procedures, possible pitfalls will be discussed. Similar strategies can be employed for other lymphoid malignancies, such as lymphoma and myeloma.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages61-77
Number of pages17
Volume2453
DOIs
Publication statusPublished - 28 May 2022

Bibliographical note

Funding Information:
We thank the members of EuroClonality and EuroMRD for their support, especially the participants of the WholeMark work package (Blanca Scheijen, Bastiaan Tops, Jan Trka, Karol Pál, Sonja Hanzelmann, Gianni Cazzaniga, Grazia Fazio, Simona Songia, and Anton W. Langerak).

Publisher Copyright:
© 2022, The Author(s).

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