Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq

V. H. J. van der Velden; L. Bastian; M. Brüggemann; A. M. Hartmann; N. Darzentas

Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq

V. H. J. van der Velden^*
, L. Bastian
, M. Brüggemann
, A. M. Hartmann
, N. Darzentas

^*Corresponding author for this work

Immunology

Research output: Chapter/Conference proceeding › Chapter › Academic

1 Citation (Scopus)

37 Downloads (Pure)

Abstract

Identification of immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements in acute lymphoblastic leukemia (ALL) patients at initial presentation are crucial for monitoring of minimal residual disease (MRD) during subsequent follow-up and thereby for appropriate risk-group stratification. Here we describe how RNA-Seq data can be generated and subsequently analyzed with ARResT/Interrogate to identify possible MRD markers. In addition to the procedures, possible pitfalls will be discussed. Similar strategies can be employed for other lymphoid malignancies, such as lymphoma and myeloma.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	61-77
Number of pages	17
Volume	2453
Publication status	Published - 28 May 2022

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© 2022, The Author(s).

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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131108809&doi=10.1007%2f978-1-0716-2115-8_4&partnerID=40&md5=7261d7f5d53e133f5623071a9853cece

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van der Velden, V. H. J., Bastian, L., Brüggemann, M., Hartmann, A. M., & Darzentas, N. (2022). Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq. In Methods in Molecular Biology (Vol. 2453, pp. 61-77) https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131108809&doi=10.1007%2f978-1-0716-2115-8_4&partnerID=40&md5=7261d7f5d53e133f5623071a9853cece

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abstract = "Identification of immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements in acute lymphoblastic leukemia (ALL) patients at initial presentation are crucial for monitoring of minimal residual disease (MRD) during subsequent follow-up and thereby for appropriate risk-group stratification. Here we describe how RNA-Seq data can be generated and subsequently analyzed with ARResT/Interrogate to identify possible MRD markers. In addition to the procedures, possible pitfalls will be discussed. Similar strategies can be employed for other lymphoid malignancies, such as lymphoma and myeloma.",

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AU - Brüggemann, M.

AU - Hartmann, A. M.

AU - Darzentas, N.

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N2 - Identification of immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements in acute lymphoblastic leukemia (ALL) patients at initial presentation are crucial for monitoring of minimal residual disease (MRD) during subsequent follow-up and thereby for appropriate risk-group stratification. Here we describe how RNA-Seq data can be generated and subsequently analyzed with ARResT/Interrogate to identify possible MRD markers. In addition to the procedures, possible pitfalls will be discussed. Similar strategies can be employed for other lymphoid malignancies, such as lymphoma and myeloma.

AB - Identification of immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements in acute lymphoblastic leukemia (ALL) patients at initial presentation are crucial for monitoring of minimal residual disease (MRD) during subsequent follow-up and thereby for appropriate risk-group stratification. Here we describe how RNA-Seq data can be generated and subsequently analyzed with ARResT/Interrogate to identify possible MRD markers. In addition to the procedures, possible pitfalls will be discussed. Similar strategies can be employed for other lymphoid malignancies, such as lymphoma and myeloma.

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M3 - Chapter

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BT - Methods in Molecular Biology

ER -

Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq

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