Immunohistochemical localization of glutathione S-transferase Alpha and Pi in human oesophageal squamous epithelium, Barrett's epithelium and carcinoma

Esther M.M. Van Lieshout, Urbain J. G. M. Van Haelst, Theo Wobbes, Wilbert H.M. Peters

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)
1 Downloads (Pure)

Abstract

High tissue levels of glutathione S-transferases (GSTs), a family of detoxification enzymes, are inversely correlated with cancer risk in the human gastrointestinal tract. Patients with Barrett's esophagus, wherein squamous epithelium is replaced by columnar epithelium, have an increased risk for developing esophageal adenocarcinoma. Biochemical analyses revealed that Barrett's epithelium contains lower levels of GST enzyme activity as well as some GST isoforms, as compared with squamous epithelium. So far, little information on the immunohistochemical distribution of the GST α and π isoforms in normal squamous epithelium, in Barrett's metaplastic epithelium or in adeno- and squamous cell carcinomas of the esophagus is available. Tissues were fixed in formalin and embedded in paraffin. Three 4 μm thick sections were used for hematoxylin and eosin staining and for immunostaining with antibodies against GST α and π. GST α and π were seen in normal squamous epithelium (0% and 75%, respectively), Barrett's epithelium (75% and 100%), adenocarcinoma (25% and 100%) and squamous cell carcinoma (27% and 91%). Staining was mainly cytoplasmic, though some nuclear staining with the GST π antibody was apparent. The varying expression of GST α and π in normal and (pre)neoplastic esophagus may have consequences for the treatment of these diseases and may contribute to an understanding of the development of these esophageal disorders.
Original languageEnglish
Pages (from-to)530-535
JournalJapanese Journal of Cancer Research
Volume90
Issue number5
DOIs
Publication statusPublished - May 1999
Externally publishedYes

Bibliographical note

ACKNOWLEDGMENTS
This work was supported by grant 94-715 from the Dutch Cancer Society.

Fingerprint

Dive into the research topics of 'Immunohistochemical localization of glutathione S-transferase Alpha and Pi in human oesophageal squamous epithelium, Barrett's epithelium and carcinoma'. Together they form a unique fingerprint.

Cite this