Implementation of flow cytometry in the diagnostic work-up of myelodysplastic syndromes in a multicenter approach: Report from the Dutch Working Party on Flow Cytometry in MDS

Theresia M. Westers*, Vincent H.J. van der Velden, Canan Alhan, Roelof Bekkema, André Bijkerk, Rik A. Brooimans, Claudia Cali, Angelika M. Dräger, Valerie de Haas, Christa Homburg, Anja de Jong, P. Ellen A. Kuiper-Kramer, Marije Leenders, Ingrid Lommerse, Jeroen G. te Marvelde, Joke K. van der Molen-Sinke, Bijan Moshaver, Andre B. Mulder, Frank W.M.B. Preijers, Roger K. SchindhelmAlita van der Sluijs, Elisabeth R. van Wering, August H. Westra, Arjan A. van de Loosdrecht

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

31 Citations (Scopus)

Abstract

Flow cytometry (FC) is recognized as an important tool in the diagnosis of myelodysplastic syndromes (MDS) especially when standard criteria fail. A working group within the Dutch Society of Cytometry aimed to implement FC in the diagnostic work-up of MDS. Hereto, guidelines for data acquisition, analysis and interpretation were formulated. Based on discussions on analyses of list mode data files and fresh MDS bone marrow samples and recent literature, the guidelines were modified. Over the years (2005-2011), the concordance between the participating centers increased indicating that the proposed guidelines contributed to a more objective, standardized FC analysis, thereby ratifying the implementation of FC in MDS.

Original languageEnglish
Pages (from-to)422-430
Number of pages9
JournalLeukemia Research
Volume36
Issue number4
DOIs
Publication statusPublished - Apr 2012

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