In Vitro Assessment of the Immunological Significance of a Human Monoclonal Antibody Directed to the Influenza A Virus Nucleoprotein

Rogier Bodewes; Tiny Mieras; J Wrammert; R Ahmed; PC Wilson; Ron Fouchier; Ab Osterhaus; Guus Rimmelzwaan

doi:10.1128/CVI.00339-13

In Vitro Assessment of the Immunological Significance of a Human Monoclonal Antibody Directed to the Influenza A Virus Nucleoprotein

Rogier Bodewes, Tiny Mieras, J Wrammert, R Ahmed, PC Wilson, Ron Fouchier, Ab Osterhaus, Guus Rimmelzwaan

Virology

Research output: Contribution to journal › Article › Academic › peer-review

30 Citations (Scopus)

Abstract

Influenza A viruses cause annual epidemics and occasionally pandemics. Antibodies directed to the conserved viral nucleoprotein (NP) may play a role in immunity against various influenza A virus subtypes. Here, we assessed the immunological significance of a human monoclonal antibody directed to NP in vitro. This antibody bound to virus-infected cells but did not display virus-neutralizing activity, complement-dependent cell cytotoxicity, or opsonization of viral antigen for improved antigen presentation to CD8(+) T cells by dendritic cells.

Original language	Undefined/Unknown
Pages (from-to)	1333-1337
Number of pages	5
Journal	Clinical and Vaccine Immunology
Volume	20
Issue number	8
DOIs	https://doi.org/10.1128/CVI.00339-13
Publication status	Published - 2013

Access to Document

10.1128/CVI.00339-13

http://hdl.handle.net/1765/68180

Cite this

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title = "In Vitro Assessment of the Immunological Significance of a Human Monoclonal Antibody Directed to the Influenza A Virus Nucleoprotein",

abstract = "Influenza A viruses cause annual epidemics and occasionally pandemics. Antibodies directed to the conserved viral nucleoprotein (NP) may play a role in immunity against various influenza A virus subtypes. Here, we assessed the immunological significance of a human monoclonal antibody directed to NP in vitro. This antibody bound to virus-infected cells but did not display virus-neutralizing activity, complement-dependent cell cytotoxicity, or opsonization of viral antigen for improved antigen presentation to CD8(+) T cells by dendritic cells.",

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pages = "1333--1337",

journal = "Clinical and Vaccine Immunology",

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publisher = "American Society for Microbiology",

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In Vitro Assessment of the Immunological Significance of a Human Monoclonal Antibody Directed to the Influenza A Virus Nucleoprotein. / Bodewes, Rogier; Mieras, Tiny; Wrammert, J; Ahmed, R; Wilson, PC; Fouchier, Ron; Osterhaus, Ab; Rimmelzwaan, Guus.

In: Clinical and Vaccine Immunology, Vol. 20, No. 8, 2013, p. 1333-1337.

Research output: Contribution to journal › Article › Academic › peer-review

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AU - Wilson, PC

AU - Fouchier, Ron

AU - Osterhaus, Ab

AU - Rimmelzwaan, Guus

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AB - Influenza A viruses cause annual epidemics and occasionally pandemics. Antibodies directed to the conserved viral nucleoprotein (NP) may play a role in immunity against various influenza A virus subtypes. Here, we assessed the immunological significance of a human monoclonal antibody directed to NP in vitro. This antibody bound to virus-infected cells but did not display virus-neutralizing activity, complement-dependent cell cytotoxicity, or opsonization of viral antigen for improved antigen presentation to CD8(+) T cells by dendritic cells.

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DO - 10.1128/CVI.00339-13

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