IN VITRO CO-CULTURE OF TISSUE-ENGINEERED MINERALIZED CARTILAGE AND VESSEL NETWORKS TO MODEL ENDOCHONDRAL OSSIFICATION

Encheng Ji; Andrea banfi; Gerjo van Osch; Janneke Bouma; Eric Farrell; Lieke Leijsten

doi:10.1302/1358-992x.2024.18.097

IN VITRO CO-CULTURE OF TISSUE-ENGINEERED MINERALIZED CARTILAGE AND VESSEL NETWORKS TO MODEL ENDOCHONDRAL OSSIFICATION

Encheng Ji^*
, Andrea banfi
, Gerjo van Osch
, Janneke Bouma
, Eric Farrell
, Lieke Leijsten

^*Corresponding author for this work

Research output: Contribution to journal › Meeting Abstract › Academic › peer-review

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Abstract

Introduction
Endochondral ossification (EO) is the process of bone development via a cartilage template. It involves multiple stages, including chondrogenesis, mineralisation and angiogenesis. Importantly, how cartilage mineralisation affects angiogenesis during EO is not fully understood. Here we aimed to develop a new in vitro co-culture model to recapitulate and study the interaction between mineralised cartilage generated from human mesenchymal stromal cells (hMSCs) and microvascular networks.
Method
Chondrogenic hMSC pellets were generated by culture with transforming growth factor (TGF)-β3. For mineralised pellets, β-glycerophosphate (BGP) was added from day 7 and TGF-β3 was withdrawn on day 14. Conditioned medium (CM) from the pellets was used to evaluate the effect on human umbilical vein endothelial cells (HUVECs) in migration, proliferation and tube …

Original language	English
Pages (from-to)	97
Number of pages	1
Journal	Bone and Joint Journal
Volume	106-B
Issue number	SUPP 18
DOIs	https://doi.org/10.1302/1358-992x.2024.18.097
Publication status	Published - 14 Nov 2024

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10.1302/1358-992x.2024.18.097

IN VITRO COAccepted author manuscript, 45.6 KBLicence: Article 25fa Dutch Copyright Act

Cite this

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title = "IN VITRO CO-CULTURE OF TISSUE-ENGINEERED MINERALIZED CARTILAGE AND VESSEL NETWORKS TO MODEL ENDOCHONDRAL OSSIFICATION",

abstract = "Introduction Endochondral ossification (EO) is the process of bone development via a cartilage template. It involves multiple stages, including chondrogenesis, mineralisation and angiogenesis. Importantly, how cartilage mineralisation affects angiogenesis during EO is not fully understood. Here we aimed to develop a new in vitro co-culture model to recapitulate and study the interaction between mineralised cartilage generated from human mesenchymal stromal cells (hMSCs) and microvascular networks. Method Chondrogenic hMSC pellets were generated by culture with transforming growth factor (TGF)-β3. For mineralised pellets, β-glycerophosphate (BGP) was added from day 7 and TGF-β3 was withdrawn on day 14. Conditioned medium (CM) from the pellets was used to evaluate the effect on human umbilical vein endothelial cells (HUVECs) in migration, proliferation and tube …",

author = "Encheng Ji and Andrea banfi and \{van Osch\}, Gerjo and Janneke Bouma and Eric Farrell and Lieke Leijsten",

year = "2024",

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doi = "10.1302/1358-992x.2024.18.097",

language = "English",

volume = "106-B",

pages = "97",

number = "SUPP 18",

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TY - JOUR

T1 - IN VITRO CO-CULTURE OF TISSUE-ENGINEERED MINERALIZED CARTILAGE AND VESSEL NETWORKS TO MODEL ENDOCHONDRAL OSSIFICATION

AU - Ji, Encheng

AU - banfi, Andrea

AU - van Osch, Gerjo

AU - Bouma, Janneke

AU - Farrell, Eric

AU - Leijsten, Lieke

PY - 2024/11/14

Y1 - 2024/11/14

N2 - Introduction Endochondral ossification (EO) is the process of bone development via a cartilage template. It involves multiple stages, including chondrogenesis, mineralisation and angiogenesis. Importantly, how cartilage mineralisation affects angiogenesis during EO is not fully understood. Here we aimed to develop a new in vitro co-culture model to recapitulate and study the interaction between mineralised cartilage generated from human mesenchymal stromal cells (hMSCs) and microvascular networks. Method Chondrogenic hMSC pellets were generated by culture with transforming growth factor (TGF)-β3. For mineralised pellets, β-glycerophosphate (BGP) was added from day 7 and TGF-β3 was withdrawn on day 14. Conditioned medium (CM) from the pellets was used to evaluate the effect on human umbilical vein endothelial cells (HUVECs) in migration, proliferation and tube …

AB - Introduction Endochondral ossification (EO) is the process of bone development via a cartilage template. It involves multiple stages, including chondrogenesis, mineralisation and angiogenesis. Importantly, how cartilage mineralisation affects angiogenesis during EO is not fully understood. Here we aimed to develop a new in vitro co-culture model to recapitulate and study the interaction between mineralised cartilage generated from human mesenchymal stromal cells (hMSCs) and microvascular networks. Method Chondrogenic hMSC pellets were generated by culture with transforming growth factor (TGF)-β3. For mineralised pellets, β-glycerophosphate (BGP) was added from day 7 and TGF-β3 was withdrawn on day 14. Conditioned medium (CM) from the pellets was used to evaluate the effect on human umbilical vein endothelial cells (HUVECs) in migration, proliferation and tube …

U2 - 10.1302/1358-992x.2024.18.097

DO - 10.1302/1358-992x.2024.18.097

M3 - Meeting Abstract

SN - 2049-4394

VL - 106-B

SP - 97

JO - Bone and Joint Journal

JF - Bone and Joint Journal

IS - SUPP 18

ER -

IN VITRO CO-CULTURE OF TISSUE-ENGINEERED MINERALIZED CARTILAGE AND VESSEL NETWORKS TO MODEL ENDOCHONDRAL OSSIFICATION

Abstract

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