In vitro sealing of iatrogenic fetal membrane defects by a collagen plug imbued with fibrinogen and plasma

A C Engels; M F Hoylaerts; M Endo; S Loyen; G Verbist; S Manodoro; P DeKoninck; J Richter; J A Deprest

doi:10.1002/pd.4032

In vitro sealing of iatrogenic fetal membrane defects by a collagen plug imbued with fibrinogen and plasma

A C Engels, M F Hoylaerts, M Endo, S Loyen, G Verbist, S Manodoro, P DeKoninck, J Richter, J A Deprest^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

OBJECTIVES:

We aimed to demonstrate local thrombin generation by fetal membranes, as well as its ability to generate fibrin from fibrinogen concentrate. Furthermore, we aimed to investigate the efficacy of collagen plugs, soaked with plasma and fibrinogen, to seal iatrogenic fetal membrane defects.

METHODS:

Thrombin generation by homogenized fetal membranes was measured by calibrated automated thrombography. To identify the coagulation caused by an iatrogenic membrane defect, we analyzed fibrin formation by optical densitometry, upon various concentrations of fibrinogen. The ability of a collagen plug soaked with fibrinogen and plasma was tested in an ex vivo model for its ability to seal an iatrogenic fetal membrane defect.

RESULTS:

Fetal membrane homogenates potently induced thrombin generation in amniotic fluid and diluted plasma. Upon the addition of fibrinogen concentrate, potent fibrin formation was triggered. Measured by densiometry, fibrin formation was optimal at 1250 µg/mL fibrinogen in combination with 4% plasma. A collagen plug soaked with fibrinogen and plasma sealed an iatrogenic membrane defect about 35% better than collagen plugs without these additives (P = 0.037).

CONCLUSIONS:

These in vitro experiments suggest that the addition of fibrinogen and plasma may enhance the sealing efficacy of collagen plugs in closing iatrogenic fetal membrane defects.

Original language	English
Pages (from-to)	162-167
Number of pages	6
Journal	Prenatal Diagnosis
Volume	33
Issue number	2
DOIs	https://doi.org/10.1002/pd.4032
Publication status	Published - Feb 2013
Externally published	Yes

Bibliographical note

© 2013 John Wiley & Sons, Ltd.

Funding sources:

Our work on tissue engineering in perinatology including membrane wound healing is funded by the European Commission (EC) via the EuroSTEC
project (LSHC-CT-2006-037409). M. E. is a beneficiary of a Marie Curie Industry-Academia Partnership and Pathways grant from the EC (251356). J. P. D. is a
beneficiary of a fundamental clinical research grant from the Fonds Wetenschappelijk Onderzoek Vlaanderen (1.8.012.07.N.02). J. R. is a beneficiary of a grant
from the KOOR Fonds of the University Hospitals Leuven.
Conflicts of interest: None declared

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title = "In vitro sealing of iatrogenic fetal membrane defects by a collagen plug imbued with fibrinogen and plasma",

abstract = "OBJECTIVES: We aimed to demonstrate local thrombin generation by fetal membranes, as well as its ability to generate fibrin from fibrinogen concentrate. Furthermore, we aimed to investigate the efficacy of collagen plugs, soaked with plasma and fibrinogen, to seal iatrogenic fetal membrane defects.METHODS: Thrombin generation by homogenized fetal membranes was measured by calibrated automated thrombography. To identify the coagulation caused by an iatrogenic membrane defect, we analyzed fibrin formation by optical densitometry, upon various concentrations of fibrinogen. The ability of a collagen plug soaked with fibrinogen and plasma was tested in an ex vivo model for its ability to seal an iatrogenic fetal membrane defect.RESULTS:Fetal membrane homogenates potently induced thrombin generation in amniotic fluid and diluted plasma. Upon the addition of fibrinogen concentrate, potent fibrin formation was triggered. Measured by densiometry, fibrin formation was optimal at 1250 µg/mL fibrinogen in combination with 4% plasma. A collagen plug soaked with fibrinogen and plasma sealed an iatrogenic membrane defect about 35% better than collagen plugs without these additives (P = 0.037).CONCLUSIONS: These in vitro experiments suggest that the addition of fibrinogen and plasma may enhance the sealing efficacy of collagen plugs in closing iatrogenic fetal membrane defects.",

author = "Engels, {A C} and Hoylaerts, {M F} and M Endo and S Loyen and G Verbist and S Manodoro and P DeKoninck and J Richter and Deprest, {J A}",

note = "{\textcopyright} 2013 John Wiley & Sons, Ltd. Funding sources: Our work on tissue engineering in perinatology including membrane wound healing is funded by the European Commission (EC) via the EuroSTEC project (LSHC-CT-2006-037409). M. E. is a beneficiary of a Marie Curie Industry-Academia Partnership and Pathways grant from the EC (251356). J. P. D. is a beneficiary of a fundamental clinical research grant from the Fonds Wetenschappelijk Onderzoek Vlaanderen (1.8.012.07.N.02). J. R. is a beneficiary of a grant from the KOOR Fonds of the University Hospitals Leuven. Conflicts of interest: None declared",

year = "2013",

month = feb,

doi = "10.1002/pd.4032",

language = "English",

volume = "33",

pages = "162--167",

journal = "Prenatal Diagnosis",

issn = "0197-3851",

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T1 - In vitro sealing of iatrogenic fetal membrane defects by a collagen plug imbued with fibrinogen and plasma

AU - Engels, A C

AU - Hoylaerts, M F

AU - Endo, M

AU - Loyen, S

AU - Verbist, G

AU - Manodoro, S

AU - DeKoninck, P

AU - Richter, J

AU - Deprest, J A

N1 - © 2013 John Wiley & Sons, Ltd. Funding sources: Our work on tissue engineering in perinatology including membrane wound healing is funded by the European Commission (EC) via the EuroSTEC project (LSHC-CT-2006-037409). M. E. is a beneficiary of a Marie Curie Industry-Academia Partnership and Pathways grant from the EC (251356). J. P. D. is a beneficiary of a fundamental clinical research grant from the Fonds Wetenschappelijk Onderzoek Vlaanderen (1.8.012.07.N.02). J. R. is a beneficiary of a grant from the KOOR Fonds of the University Hospitals Leuven. Conflicts of interest: None declared

PY - 2013/2

Y1 - 2013/2

N2 - OBJECTIVES: We aimed to demonstrate local thrombin generation by fetal membranes, as well as its ability to generate fibrin from fibrinogen concentrate. Furthermore, we aimed to investigate the efficacy of collagen plugs, soaked with plasma and fibrinogen, to seal iatrogenic fetal membrane defects.METHODS: Thrombin generation by homogenized fetal membranes was measured by calibrated automated thrombography. To identify the coagulation caused by an iatrogenic membrane defect, we analyzed fibrin formation by optical densitometry, upon various concentrations of fibrinogen. The ability of a collagen plug soaked with fibrinogen and plasma was tested in an ex vivo model for its ability to seal an iatrogenic fetal membrane defect.RESULTS:Fetal membrane homogenates potently induced thrombin generation in amniotic fluid and diluted plasma. Upon the addition of fibrinogen concentrate, potent fibrin formation was triggered. Measured by densiometry, fibrin formation was optimal at 1250 µg/mL fibrinogen in combination with 4% plasma. A collagen plug soaked with fibrinogen and plasma sealed an iatrogenic membrane defect about 35% better than collagen plugs without these additives (P = 0.037).CONCLUSIONS: These in vitro experiments suggest that the addition of fibrinogen and plasma may enhance the sealing efficacy of collagen plugs in closing iatrogenic fetal membrane defects.

AB - OBJECTIVES: We aimed to demonstrate local thrombin generation by fetal membranes, as well as its ability to generate fibrin from fibrinogen concentrate. Furthermore, we aimed to investigate the efficacy of collagen plugs, soaked with plasma and fibrinogen, to seal iatrogenic fetal membrane defects.METHODS: Thrombin generation by homogenized fetal membranes was measured by calibrated automated thrombography. To identify the coagulation caused by an iatrogenic membrane defect, we analyzed fibrin formation by optical densitometry, upon various concentrations of fibrinogen. The ability of a collagen plug soaked with fibrinogen and plasma was tested in an ex vivo model for its ability to seal an iatrogenic fetal membrane defect.RESULTS:Fetal membrane homogenates potently induced thrombin generation in amniotic fluid and diluted plasma. Upon the addition of fibrinogen concentrate, potent fibrin formation was triggered. Measured by densiometry, fibrin formation was optimal at 1250 µg/mL fibrinogen in combination with 4% plasma. A collagen plug soaked with fibrinogen and plasma sealed an iatrogenic membrane defect about 35% better than collagen plugs without these additives (P = 0.037).CONCLUSIONS: These in vitro experiments suggest that the addition of fibrinogen and plasma may enhance the sealing efficacy of collagen plugs in closing iatrogenic fetal membrane defects.

U2 - 10.1002/pd.4032

DO - 10.1002/pd.4032

M3 - Article

C2 - 23296773

SN - 0197-3851

VL - 33

SP - 162

EP - 167

JO - Prenatal Diagnosis

JF - Prenatal Diagnosis

IS - 2

ER -

In vitro sealing of iatrogenic fetal membrane defects by a collagen plug imbued with fibrinogen and plasma

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