TY - JOUR
T1 - In vivo experiments with mesothelial cell seeded ePTFE vascular grafts
AU - Verhagen, H. J.M.
AU - Blankensteijn, J. D.
AU - De Groot, Ph G.
AU - Heijnen-Snyder, G. J.
AU - Pronk, A.
AU - Vroom, Th M.
AU - Muller, H. J.
AU - Nicolay, K.
AU - Van Vroonhoven, Th J.M.V.
AU - Sixma, J. J.
AU - Eikelboom, B. C.
N1 - Funding Information:
This research was partially supported by GNSAGA of CNR.
PY - 1998/6
Y1 - 1998/6
N2 - Objectives: To investigate the influence of mesothelial cell (MC) seeding on patency and neointimal formation of small diameter ePTFE grafts in a canine model. Materials and Methods: MC were isolated from the omentum, cultured, seeded on fibronectin-coated ePTFE grafts (4 cm, 4 mm ID), and implanted in the carotid artery of five Beagle dogs. Each dog also received a non-seeded control graft. Patency was assessed by palpation immediately after implantation, and non-invasively by magnetic resonance angiography (MRA) after 1 week and just prior to sacrifice (4 weeks). Intimal thickness was quantified on histological sections by use of computer-aided morphometry. Results: All grafts were patent after implantation. After 1 week, MRA showed the loss of lumen diameter in two seeded grafts. After 4 weeks, two seeded grafts were occluded, one seeded graft was severely stenosed, and all others were without angiographic lumen reduction. Histology and morphometry confirmed that two seeded grafts were occluded, and demonstrated that the other three seeded grafts showed significantly more intima information (0.22-1.34 mm) than the control grafts (< 0.08 mm; p < 0.01). Conclusions: The MC seeding process decreases patency and increases neointimal formation of small diameter ePTFE grafts in dogs and does not seem to be useful for reduction of graft thrombogenicity.
AB - Objectives: To investigate the influence of mesothelial cell (MC) seeding on patency and neointimal formation of small diameter ePTFE grafts in a canine model. Materials and Methods: MC were isolated from the omentum, cultured, seeded on fibronectin-coated ePTFE grafts (4 cm, 4 mm ID), and implanted in the carotid artery of five Beagle dogs. Each dog also received a non-seeded control graft. Patency was assessed by palpation immediately after implantation, and non-invasively by magnetic resonance angiography (MRA) after 1 week and just prior to sacrifice (4 weeks). Intimal thickness was quantified on histological sections by use of computer-aided morphometry. Results: All grafts were patent after implantation. After 1 week, MRA showed the loss of lumen diameter in two seeded grafts. After 4 weeks, two seeded grafts were occluded, one seeded graft was severely stenosed, and all others were without angiographic lumen reduction. Histology and morphometry confirmed that two seeded grafts were occluded, and demonstrated that the other three seeded grafts showed significantly more intima information (0.22-1.34 mm) than the control grafts (< 0.08 mm; p < 0.01). Conclusions: The MC seeding process decreases patency and increases neointimal formation of small diameter ePTFE grafts in dogs and does not seem to be useful for reduction of graft thrombogenicity.
UR - http://www.scopus.com/inward/record.url?scp=0031850684&partnerID=8YFLogxK
U2 - 10.1016/S1078-5884(98)80108-1
DO - 10.1016/S1078-5884(98)80108-1
M3 - Article
C2 - 9659883
AN - SCOPUS:0031850684
SN - 1078-5884
VL - 15
SP - 489
EP - 496
JO - European Journal of Vascular and Endovascular Surgery
JF - European Journal of Vascular and Endovascular Surgery
IS - 6
ER -