In vivo Raman spectroscopy detects increased epidermal antioxidative potential with topically applied carotenoids

Juergen Lademann*, P. J. Caspers, A. van der Pol, H. Richter, A. Patzelt, L. Zastrow, M. Darvin, W. Sterry, J. W. Fluhr

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

In the present study, the distribution of the carotenoids as a marker for the complete antioxidative potential in human skin was investigated before and after the topical application of carotenoids by in vivo Raman spectroscopy with an excitation wavelength of 785 nm. The carotenoid profile was assessed after a short term topical application in 4 healthy volunteers. In the untreated skin, the highest concentration of natural carotenoids was detected in different layers of the stratum corneum (SC) close to the skin surface. After topical application of carotenoids, an increase in the antioxidative potential in the skin could be observed. Topically applied carotenoids penetrate deep into the epidermis down to approximately 24 μm. This study supports the hypothesis that antioxidative substances are secreted via eccrine sweat glands and/or sebaceous glands to the skin surface. Subsequently they penetrate into the different layers of the SC. A graph is presented. Typical Raman spectrum of untreated skin (forearm, 10 μm depth) under the excitation wavelength of 785 nm.

Original languageEnglish
Pages (from-to)76-79
Number of pages4
JournalLaser Physics Letters
Volume6
Issue number1
DOIs
Publication statusPublished - Jan 2009

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