Increased Sperm DNA Fragmentation in Patients With Vasectomy Reversal Has No Prognostic Value for Pregnancy Rate

Mandy Smit, OG (Odette) Wissenburg, Hans Romijn, Gert Dohle

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

Purpose: We evaluated sperm DNA fragmentation in patients with vasectomy reversal and its prognostic value to determine spontaneous and assisted reproductive technique pregnancy rates. Materials and Methods: We prospectively assessed DNA fragmentation with the sperm chromatin structure assay in postoperative semen samples of 70 patients with vasectomy reversal. At a median +/- SD followup of 4.3 +/- 0.5 years pregnancy rates were recorded. ResuIts: DNA fragmentation in patients with vasectomy reversal was significantly increased vs that in proven fertile. controls (30.2% +/- 20.1% vs 15.3% +/- 5.4%, p <0.001). Significant negative correlations were found between DNA fragmentation index and total sperm count, progressive motility, total number of progressive sperm, normal morphology and sperm vitality (-0.325 <r <-0.805). The obstructive interval did not correlate with DNA fragmentation. The spontaneous pregnancy rate was 46%. Significantly higher log total progressive sperm motility (p = 0.021) and a trend toward lower female age (p = 0.064) were detected in the spontaneous vs the no pregnancy group. No association was found between DNA fragmentation and the pregnancy rate. Conclusions: Increased DNA fragmentation is present in semen samples of men after vasectomy reversal vs fertile controls but DNA fragmentation is not associated with spontaneous or assisted reproductive technique pregnancy rates in these patients.
Original languageUndefined/Unknown
Pages (from-to)662-665
Number of pages4
JournalJournal of Urology
Volume183
Issue number2
DOIs
Publication statusPublished - 2010

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