Independent and interrelated regulation of ornithine decarboxylase by calcium and cAMP in fetal rat osteoblasts

J. P.T.M. van Leeuwen; M. P. Bos; M. P.M. Herrmann-Erlee

doi:10.1016/0143-4160(88)90022-X

Independent and interrelated regulation of ornithine decarboxylase by calcium and cAMP in fetal rat osteoblasts

J. P.T.M. van Leeuwen^*
, M. P. Bos
, M. P.M. Herrmann-Erlee

^*Corresponding author for this work

Leiden University

Research output: Contribution to journal › Article › Academic › peer-review

8 Citations (Scopus)

Abstract

The present study was undertaken to determine in fetal rat osteoblasts whether and how the intracellular messengers calcium and cAMP are involved in stimulation of ornithine decarboxylase (ODC) activity. For that purpose we used different drugs affecting [Ca²⁺]_i and cAMP concentration. A23187 stimulates ODC activity in a biphasic way, with maximal stimulation at 100 nM A23187. At that concentration no stimulation of cAMP production was observed. Basal and A23187-stimulated (100 nM) ODC activity were inhibited by EGTA and trifluoperazine. Forskolin stimulated dose-dependently both ODC activity and cAMP production. Besides these effects forskolin (1 and 10 μM) increased the [Ca²⁺]_i via an increased calcium influx. Addition of La³⁺, verapamil or EGTA, but not of trifluoperazine, significantly inhibited the forskolin-stimulated (10 μM) ODC activity. When forskolin (100 nM and 1 μM) was added together with 1 μM A23187, a synergistic stimulation of ODC activity was observed. These results implicate that calcium is involved in basal ODC activity, and that ODC activity can be stimulated via (1) a cAMP-independent calcium pathway, and (2) a calcium-dependent, cAMP pathway. It is proposed that ODC activity can be stimulated via interaction between calcium and cAMP.

Original language	English
Pages (from-to)	181-191
Number of pages	11
Journal	Cell Calcium
Volume	9
Issue number	4
DOIs	https://doi.org/10.1016/0143-4160(88)90022-X
Publication status	Published - Aug 1988
Externally published	Yes

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10.1016/0143-4160(88)90022-X

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title = "Independent and interrelated regulation of ornithine decarboxylase by calcium and cAMP in fetal rat osteoblasts",

abstract = "The present study was undertaken to determine in fetal rat osteoblasts whether and how the intracellular messengers calcium and cAMP are involved in stimulation of ornithine decarboxylase (ODC) activity. For that purpose we used different drugs affecting [Ca2+]i and cAMP concentration. A23187 stimulates ODC activity in a biphasic way, with maximal stimulation at 100 nM A23187. At that concentration no stimulation of cAMP production was observed. Basal and A23187-stimulated (100 nM) ODC activity were inhibited by EGTA and trifluoperazine. Forskolin stimulated dose-dependently both ODC activity and cAMP production. Besides these effects forskolin (1 and 10 μM) increased the [Ca2+]i via an increased calcium influx. Addition of La3+, verapamil or EGTA, but not of trifluoperazine, significantly inhibited the forskolin-stimulated (10 μM) ODC activity. When forskolin (100 nM and 1 μM) was added together with 1 μM A23187, a synergistic stimulation of ODC activity was observed. These results implicate that calcium is involved in basal ODC activity, and that ODC activity can be stimulated via (1) a cAMP-independent calcium pathway, and (2) a calcium-dependent, cAMP pathway. It is proposed that ODC activity can be stimulated via interaction between calcium and cAMP.",

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year = "1988",

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AU - van Leeuwen, J. P.T.M.

AU - Bos, M. P.

AU - Herrmann-Erlee, M. P.M.

PY - 1988/8

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N2 - The present study was undertaken to determine in fetal rat osteoblasts whether and how the intracellular messengers calcium and cAMP are involved in stimulation of ornithine decarboxylase (ODC) activity. For that purpose we used different drugs affecting [Ca2+]i and cAMP concentration. A23187 stimulates ODC activity in a biphasic way, with maximal stimulation at 100 nM A23187. At that concentration no stimulation of cAMP production was observed. Basal and A23187-stimulated (100 nM) ODC activity were inhibited by EGTA and trifluoperazine. Forskolin stimulated dose-dependently both ODC activity and cAMP production. Besides these effects forskolin (1 and 10 μM) increased the [Ca2+]i via an increased calcium influx. Addition of La3+, verapamil or EGTA, but not of trifluoperazine, significantly inhibited the forskolin-stimulated (10 μM) ODC activity. When forskolin (100 nM and 1 μM) was added together with 1 μM A23187, a synergistic stimulation of ODC activity was observed. These results implicate that calcium is involved in basal ODC activity, and that ODC activity can be stimulated via (1) a cAMP-independent calcium pathway, and (2) a calcium-dependent, cAMP pathway. It is proposed that ODC activity can be stimulated via interaction between calcium and cAMP.

AB - The present study was undertaken to determine in fetal rat osteoblasts whether and how the intracellular messengers calcium and cAMP are involved in stimulation of ornithine decarboxylase (ODC) activity. For that purpose we used different drugs affecting [Ca2+]i and cAMP concentration. A23187 stimulates ODC activity in a biphasic way, with maximal stimulation at 100 nM A23187. At that concentration no stimulation of cAMP production was observed. Basal and A23187-stimulated (100 nM) ODC activity were inhibited by EGTA and trifluoperazine. Forskolin stimulated dose-dependently both ODC activity and cAMP production. Besides these effects forskolin (1 and 10 μM) increased the [Ca2+]i via an increased calcium influx. Addition of La3+, verapamil or EGTA, but not of trifluoperazine, significantly inhibited the forskolin-stimulated (10 μM) ODC activity. When forskolin (100 nM and 1 μM) was added together with 1 μM A23187, a synergistic stimulation of ODC activity was observed. These results implicate that calcium is involved in basal ODC activity, and that ODC activity can be stimulated via (1) a cAMP-independent calcium pathway, and (2) a calcium-dependent, cAMP pathway. It is proposed that ODC activity can be stimulated via interaction between calcium and cAMP.

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U2 - 10.1016/0143-4160(88)90022-X

DO - 10.1016/0143-4160(88)90022-X

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AN - SCOPUS:0023688178

SN - 0143-4160

VL - 9

SP - 181

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JO - Cell Calcium

JF - Cell Calcium

IS - 4

ER -

Independent and interrelated regulation of ornithine decarboxylase by calcium and cAMP in fetal rat osteoblasts

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