Inducible HIV-1 Reservoir Quantification: Clinical Relevance, Applications and Advancements of TILDA

Cynthia Lungu, Riddhima Banga, Rob A. Gruters, Francesco A. Procopio*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)
4 Downloads (Pure)

Abstract

The presence of a stable HIV-1 reservoir persisting over time despite effective antiretroviral suppression therapy precludes a cure for HIV-1. Characterizing and quantifying this residual reservoir is considered an essential prerequisite to develop and validate curative strategies. However, a sensitive, reproducible, cost-effective, and easily executable test is still needed. The quantitative viral outgrowth assay is considered the gold standard approach to quantify the reservoir in HIV-1-infected patients on suppressive ART, but it has several limitations. An alternative method to quantify the viral reservoir following the reactivation of latent HIV-1 provirus detects multiply-spliced tat/rev RNA (msRNA) molecules by real-time PCR [tat/rev induced limiting dilution assay (TILDA)]. This article provides a perspective overview of the clinical relevance, various applications, recent advancements of TILDA, and how the assay has contributed to our understanding of the HIV-1 reservoir.

Original languageEnglish
Article number686690
JournalFrontiers in Microbiology
Volume12
DOIs
Publication statusPublished - 15 Jun 2021

Bibliographical note

Funding Information:
This work was supported by EHVA, funded by the European Union’s Horizon 2020 Research and Innovation Program (grant number 681032) and the Swiss Government (grant number 150337).

Publisher Copyright:
© Copyright © 2021 Lungu, Banga, Gruters and Procopio.

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