Induction of Staphylococcus aureus-specific IgA and agglutination potency in milk of cows by mucosal immunization

MJT Plat-Sinnige, Nelianne Verkaik, Willem van Wamel, N (Natasja) de Groot, DS Acton, Alex Belkum

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Lactating cows were immunized with inactivated Staphylococcus aureus strains and concentrated culture supernatants. Application of a repeated mucosal immunization scheme resulted in significant levels of S. aureus-specific IgA in milk of dairy cows. Average IgA titers against whole cell S. aureus increased during the first 10 weeks of immunization after which a plateau level was reached and maintained during lactation. Immune whey agglutinated both bovine and human S. aureus strains including methicillin-resistant S. aureus (MRSA) strains and recognized extracted S. aureus proteins on Western blot. ELISAs to quantify milk IgA reactive with a number of S. aureus virulence proteins (e.g. enterotoxins, microbial surface component recognizing adhesive matrix molecules (MSCRAMMs) and immune modulating proteins) and cell wall components, demonstrated the polyclonality of the IgA. Correlations observed between agglutination and specific IgA titers for whey and for purified IgA suggested functionality of the induced antibodies. Milk from immunized cows may provide a way of producing potentially therapeutic polyclonal antibodies against S. aureus colonization and infection. (C) 2009 Elsevier Ltd. All rights reserved.
Original languageUndefined/Unknown
Pages (from-to)4001-4009
Number of pages9
JournalVaccine
Volume27
Issue number30
DOIs
Publication statusPublished - 2009

Cite this