Inhibition of OATP1B1 by tyrosine kinase inhibitors: in vitro-in vivo correlations

S Hu, Ron Mathijssen, Peter de Bruijn, SD Baker, A Sparreboom

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Abstract

Background: Several tyrosine kinase inhibitors (TKIs) can decrease docetaxel clearance in patients by an unknown mechanism. We hypothesised that these interactions are mediated by the hepatic uptake transporter OATP1B1. Methods: The influence of 16 approved TKIs on transport was studied in vitro using HEK293 cells expressing OATP1B1 or its mouse equivalent Oatp1b2. Pharmacokinetic studies were performed with Oatp1b2-knockout and OATP1B1-transgenic mice. Results: All docetaxel-interacting TKIs, including sorafenib, were identified as potent inhibitors of OATP1B1 in vitro. Although Oatp1b2 deficiency in vivo was associated with increased docetaxel exposure, single-or multiple-dose sorafenib did not influence docetaxel pharmacokinetics. Conclusion: These findings highlight the importance of identifying proper preclinical models for verifying and predicting TKI-chemotherapy interactions involving transporters.
Original languageUndefined/Unknown
Pages (from-to)894-898
Number of pages5
JournalBritish Journal of Cancer
Volume110
Issue number4
DOIs
Publication statusPublished - 2014

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