TY - JOUR
T1 - Interactions between cytokines and eicosanoids
T2 - a study using human peritoneal macrophages
AU - Pruimboom, Wanda M.
AU - van Dijk, Jeanette A.P.M.
AU - Tak, CornéJ J.A.M.
AU - Garrelds, Ingrid
AU - Bonta, Ivan L.
AU - Wilson, Paul J.H.
AU - Zijlstra, Freek J.
PY - 1994/7
Y1 - 1994/7
N2 - To examine the interactions between the main proinflammatory cytokines and eicosanoids produced by human inflammatory cells, human peritoneal macrophages (hp-Mφ) were isolated from ascitic fluid of patients with portal hypertension. Interactions between interleukin-1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α), leukotriene B4 (LTB4) and prostaglandin E2 (PGE2) were studied by addition or inhibition of several cytokines and eicosanoids: human recombinant IL-1β (hrIL-1β) addition, LTB4 addition and 5-lipoxygenase inhibition (6-hydroxy-2-(4-sulfamoylbenzylamino)-4,5,7-trimethylbenzothiazole hydrochloride (E6080)), PGE2 addition and cyclooxygenase inhibition (indomethacin). In hp-Mφ hrIL-1β stimulated the LTB4 production, while the PGE2 production was inhibited. HrIL-1β had no significant effect on IL-6 production in hp-Mφ. LTB4 did not regulate IL-1β and IL-6 production. Increasing PGE2 down regulated the TNF-α production, but did not effect the IL-1β and IL-6 production.
AB - To examine the interactions between the main proinflammatory cytokines and eicosanoids produced by human inflammatory cells, human peritoneal macrophages (hp-Mφ) were isolated from ascitic fluid of patients with portal hypertension. Interactions between interleukin-1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α), leukotriene B4 (LTB4) and prostaglandin E2 (PGE2) were studied by addition or inhibition of several cytokines and eicosanoids: human recombinant IL-1β (hrIL-1β) addition, LTB4 addition and 5-lipoxygenase inhibition (6-hydroxy-2-(4-sulfamoylbenzylamino)-4,5,7-trimethylbenzothiazole hydrochloride (E6080)), PGE2 addition and cyclooxygenase inhibition (indomethacin). In hp-Mφ hrIL-1β stimulated the LTB4 production, while the PGE2 production was inhibited. HrIL-1β had no significant effect on IL-6 production in hp-Mφ. LTB4 did not regulate IL-1β and IL-6 production. Increasing PGE2 down regulated the TNF-α production, but did not effect the IL-1β and IL-6 production.
UR - https://www.scopus.com/pages/publications/0027992274
U2 - 10.1016/0165-2478(94)90142-2
DO - 10.1016/0165-2478(94)90142-2
M3 - Article
C2 - 8002047
AN - SCOPUS:0027992274
SN - 0165-2478
VL - 41
SP - 255
EP - 260
JO - Immunology Letters
JF - Immunology Letters
IS - 2-3
ER -