Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

S F Hurst; S E Kidd; C O Morrissey; E Snelders; W J G Melchers; M V Castelli; E Mellado; K Simmon; C A Petti; S Richardson; S Zhang; A M Romanelli; B L Wickes; H A de Valk; C H W Klaassen; S Arunmozhi Balajee

doi:10.1128/JCM.00124-09

Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

S F Hurst, S E Kidd, C O Morrissey, E Snelders, W J G Melchers, M V Castelli, E Mellado, K Simmon, C A Petti, S Richardson, S Zhang, A M Romanelli, B L Wickes, H A de Valk, C H W Klaassen, S Arunmozhi Balajee

Research output: Contribution to journal › Article › Academic › peer-review

14 Citations (Scopus)

Abstract

Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

Original language	English
Pages (from-to)	1562-4
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	47
Issue number	5
DOIs	https://doi.org/10.1128/JCM.00124-09
Publication status	Published - May 2009
Externally published	Yes

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Hurst, S. F., Kidd, S. E., Morrissey, C. O., Snelders, E., Melchers, W. J. G., Castelli, M. V., Mellado, E., Simmon, K., Petti, C. A., Richardson, S., Zhang, S., Romanelli, A. M., Wickes, B. L., de Valk, H. A., Klaassen, C. H. W., & Balajee, S. A. (2009). Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus. Journal of Clinical Microbiology, 47(5), 1562-4. https://doi.org/10.1128/JCM.00124-09

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title = "Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus",

abstract = "Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.",

author = "Hurst, {S F} and Kidd, {S E} and Morrissey, {C O} and E Snelders and Melchers, {W J G} and Castelli, {M V} and E Mellado and K Simmon and Petti, {C A} and S Richardson and S Zhang and Romanelli, {A M} and Wickes, {B L} and {de Valk}, {H A} and Klaassen, {C H W} and Balajee, {S Arunmozhi}",

year = "2009",

month = may,

doi = "10.1128/JCM.00124-09",

language = "English",

volume = "47",

pages = "1562--4",

journal = "Journal of Clinical Microbiology",

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publisher = "American Society for Microbiology",

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Hurst, SF, Kidd, SE, Morrissey, CO, Snelders, E, Melchers, WJG, Castelli, MV, Mellado, E, Simmon, K, Petti, CA, Richardson, S, Zhang, S, Romanelli, AM, Wickes, BL, de Valk, HA, Klaassen, CHW & Balajee, SA 2009, 'Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus', Journal of Clinical Microbiology, vol. 47, no. 5, pp. 1562-4. https://doi.org/10.1128/JCM.00124-09

TY - JOUR

T1 - Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

AU - Hurst, S F

AU - Kidd, S E

AU - Morrissey, C O

AU - Snelders, E

AU - Melchers, W J G

AU - Castelli, M V

AU - Mellado, E

AU - Simmon, K

AU - Petti, C A

AU - Richardson, S

AU - Zhang, S

AU - Romanelli, A M

AU - Wickes, B L

AU - de Valk, H A

AU - Klaassen, C H W

AU - Balajee, S Arunmozhi

PY - 2009/5

Y1 - 2009/5

N2 - Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

AB - Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

U2 - 10.1128/JCM.00124-09

DO - 10.1128/JCM.00124-09

M3 - Article

C2 - 19261791

SN - 0095-1137

VL - 47

SP - 1562

EP - 1564

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 5

ER -

Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

Abstract

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