Interleukin-5 and eosinophil cationic protein in nasal lavages of rhinitis patients

Ingrid M. Garrelds*, Tineke De Graaf-in 't Veld, Marie Anne Nahori, B. Boris Vargaftig, Roy Gerth van Wijk, Freek J. Zijlstra

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

32 Citations (Scopus)

Abstract

The production of interleukin-5 and eosinophil cationic protein (ECP) in the nasal cavity was examined in 24 patients with rhinitis who were allergic to the house dust mite. During a double-blind placebo-controlled cross-over study, fluticasone propionate aqueous nasal spray (200 μg) was administered twice daily for 2 weeks. After four basal nasal lavages provocation with house dust mite extract was performed and nasal lavages were collected every hour for 9.5 h. Interleukin-5 was present in detectable amounts in nasal lavages from patients allergic to house dust mite. Nasal challenge with house dust mite extract caused immediate nasal symptoms and increased levels of interleukin-5. Between 3.5 and 8.5 h after the challenge symptoms recurred and interleukin-5 levels increased, reflecting a late phase reaction. Eosinophil cationic protein, a marker of activated eosinophils, was released between 6.5 and 9.5 h after challenge. Treatment with fluticasone propionate (as an aqueous nasal spray) significantly decreased the evoked interleukin-5 and ECP levels in the late phase reaction. This response was correlated with an improved symptom score. This could indicate that the number and activity of eosinophils are increased during the late phase allergic reaction, a response that is inhibited by corticosteroids.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalEuropean Journal of Pharmacology
Volume275
Issue number3
DOIs
Publication statusPublished - 14 Mar 1995

Bibliographical note

Funding Information: This study was supported by Glaxo BV, Netherlands and by the Netherlands Asthma Foundation, Grant 32.92.74. Fluticasone propionate aqueous nasal spray was given by Glaxo.

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