Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

Paola L Carvajal Monroy; Zipora Yablonka-Reuveni; Sander Grefte; Anne Marie Kuijpers-Jagtman; Frank A D T G Wagener; Johannes W Von den Hoff

doi:10.3791/52802

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

Paola L Carvajal Monroy, Zipora Yablonka-Reuveni, Sander Grefte, Anne Marie Kuijpers-Jagtman, Frank A D T G Wagener, Johannes W Von den Hoff

Oral and Maxillofacial Surgery

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

Fibrosis and defective muscle regeneration can hamper the functional recovery of the soft palate muscles after cleft palate repair. This causes persistent problems in speech, swallowing, and sucking. In vitro culture systems that allow the study of satellite cells (myogenic stem cells) from head muscles are crucial to develop new therapies based on tissue engineering to promote muscle regeneration after surgery. These systems will offer new perspectives for the treatment of cleft palate patients. A protocol for the isolation, culture and differentiation of satellite cells from head muscles is presented. The isolation is based on enzymatic digestion and trituration to release the satellite cells. In addition, this protocol comprises an innovative method using extracellular matrix gel coatings of millimeter size, which requires only low numbers of satellite cells for differentiation assays.

Original language	English
Pages (from-to)	e52802
Journal	Journal of Visualized Experiments
Issue number	101
DOIs	https://doi.org/10.3791/52802
Publication status	Published - 20 Jul 2015

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title = "Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles",

abstract = "Fibrosis and defective muscle regeneration can hamper the functional recovery of the soft palate muscles after cleft palate repair. This causes persistent problems in speech, swallowing, and sucking. In vitro culture systems that allow the study of satellite cells (myogenic stem cells) from head muscles are crucial to develop new therapies based on tissue engineering to promote muscle regeneration after surgery. These systems will offer new perspectives for the treatment of cleft palate patients. A protocol for the isolation, culture and differentiation of satellite cells from head muscles is presented. The isolation is based on enzymatic digestion and trituration to release the satellite cells. In addition, this protocol comprises an innovative method using extracellular matrix gel coatings of millimeter size, which requires only low numbers of satellite cells for differentiation assays. ",

author = "{Carvajal Monroy}, {Paola L} and Zipora Yablonka-Reuveni and Sander Grefte and Kuijpers-Jagtman, {Anne Marie} and Wagener, {Frank A D T G} and {Von den Hoff}, {Johannes W}",

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T1 - Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

AU - Carvajal Monroy, Paola L

AU - Yablonka-Reuveni, Zipora

AU - Grefte, Sander

AU - Kuijpers-Jagtman, Anne Marie

AU - Wagener, Frank A D T G

AU - Von den Hoff, Johannes W

PY - 2015/7/20

Y1 - 2015/7/20

N2 - Fibrosis and defective muscle regeneration can hamper the functional recovery of the soft palate muscles after cleft palate repair. This causes persistent problems in speech, swallowing, and sucking. In vitro culture systems that allow the study of satellite cells (myogenic stem cells) from head muscles are crucial to develop new therapies based on tissue engineering to promote muscle regeneration after surgery. These systems will offer new perspectives for the treatment of cleft palate patients. A protocol for the isolation, culture and differentiation of satellite cells from head muscles is presented. The isolation is based on enzymatic digestion and trituration to release the satellite cells. In addition, this protocol comprises an innovative method using extracellular matrix gel coatings of millimeter size, which requires only low numbers of satellite cells for differentiation assays.

AB - Fibrosis and defective muscle regeneration can hamper the functional recovery of the soft palate muscles after cleft palate repair. This causes persistent problems in speech, swallowing, and sucking. In vitro culture systems that allow the study of satellite cells (myogenic stem cells) from head muscles are crucial to develop new therapies based on tissue engineering to promote muscle regeneration after surgery. These systems will offer new perspectives for the treatment of cleft palate patients. A protocol for the isolation, culture and differentiation of satellite cells from head muscles is presented. The isolation is based on enzymatic digestion and trituration to release the satellite cells. In addition, this protocol comprises an innovative method using extracellular matrix gel coatings of millimeter size, which requires only low numbers of satellite cells for differentiation assays.

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DO - 10.3791/52802

M3 - Article

C2 - 26274878

SN - 1940-087X

SP - e52802

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

IS - 101

ER -

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

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