Isolation and mapping of human chromosome 21 cosmids using a probe for rtvl-h retrovirus-like elements

Ingrid Mieulenbelt, Martin C. Wapenaar, David Patterson, Jan Vug, Andre G. Uitterlinden*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

We have explored the usefulness of a cloned low-repetitive DNA element, termed RTVL-H, for the region-specific isolation of DNA cosmids from chromosome 21. Hybridization of the RTVL-H element as a probe to genomic Southern blots of DNAs from a chromosome 21-specific somatic cell hybrid panel demonstrated the presence of at least seven RTVL-H-related sequences in defined regions dispersed over the chromosome. Some of the RTVL-H sequences detected by using the chromosome 21-specific somatic cell hybrid panel were subsequently isolated from a chromosome 21-specific cosmid library and mapped using somatic cell hybrids. In general, close correspondence was found between mapping results obtained using the consensus RTVL-H probe on the chromosome 21-specific somatic cell hybrid panel and the more precise mapping obtained using cosmids. The results demonstrate that sequences such as the RTVL-H element can be used for isolating region-specific sequences.

Original languageEnglish
Pages (from-to)492-499
Number of pages8
JournalGenomics
Volume15
Issue number3
DOIs
Publication statusPublished - 1 Jan 1993
Externally publishedYes

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