Kompleksowa terapia komórkowo-genowa w leczeniu krytycznego niedokrwienia kończyn dolnych

Translated title of the contribution: Complex gene-cell therapy in treatment of critical lower limbs ischemia

Jan Skóra*, Artur Pupka, Piotr Barć, PrzemyŁsaw Szyber, Wojciech Polak, Piotr Szyber

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

The aim. The paper presents the results of our hybrid gene-cell therapy in case of critical lower limb ischemia. Eighteen patients with critical limb ischemia were enrolled to the study. The bone marrow from each patient was harvested. It had been incubated with VEGF165 gene plasmid for two hours before it was administrated intramuscularly into patient's lower limb. Methods. In the study we evaluated: safety, clinical outcomes of the therapy, venous blood VEGF protein concentration before and after (at: 7th, 14th, 28th and 90th day) administration of the stem cells. We obtained samples of muscles from the patients who underwent the limb amputation, which were examined histological and by PCR (Polymerase Chain Reaction) for detection of plasmid genes. Results. We observed very good clinical outcomes of the therapy. In ten of eighteen patients (∼55%) critical lower limb ischemia symptoms subsided - they saved their limbs. The serum VEGF concentration was higher than in healthy controls (p<0.05). We observed the increase of the concentration of the cytokine at the 14 th day an decrease at the 90th day after administration of cells with plasmid. We found expression of plasmid VEGF in transfected stem cells and in tissues taken from amputated limbs. However histological examination did not reveal any sings of new blood vessels formation in the samples taken from ischemie limbs. Conclusions. The therapy is safe and effective. We observed significant improvement in patient's clinical state. The therapy needs further investigations.

Translated title of the contributionComplex gene-cell therapy in treatment of critical lower limbs ischemia
Original languageUndefined/Unknown
Pages (from-to)121-125
Number of pages5
JournalPolski Merkuriusz Lekarski
Volume22
Issue number128
Publication statusPublished - 2007
Externally publishedYes

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