Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy

Mirjam Visscher, Miguel A. Pleitez*, Kim Van Gaalen, Ingeborg M. Nieuwenhuizen-Bakker, Vasilis Ntziachristos, Gijs Van Soest

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Background and aims: Analysis of atherosclerotic plaque composition is a vital tool for unraveling the pathological metabolic processes that contribute to plaque growth. Methods: We visualize the constitution of human carotid plaques by mid-infrared optoacoustic microscopy (MiROM), a method for label-free analytic histology that requires minimal tissue preparation, rapidly yielding large field-of-view en-face images with a resolution of a few micrometers. We imaged endarterectomy specimens (n = 3, 12 sections total) at specific vibrational modes, targeting carbohydrates, lipids and proteins. Additionally, we recorded spectra at selected tissue locations. We identified correlations in the variability in this high-dimensional data set using non-negative matrix factorization (NMF). Results: We visualized high-risk plaque features with molecular assignment. Consistent NMF components relate to different dominant tissue constituents, dominated by lipids, proteins, and cholesterol and carbohydrates respectively. Conclusions: These results introduce MiROM as an innovative, stain-free, analytic histology technology for the biochemical characterization of complex human vascular pathology.

Original languageEnglish
Article number100354
Publication statusPublished - Jun 2022

Bibliographical note

Funding Information:
This work is supported by Stichting Fonds Dr. Catharine van Tussenbroek and Nederlandse Organisatie voor Wetenschappelijk Onderzoek , project number: Vici 16131 .

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© 2022 The Authors


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