Label-free single-cell RNA multiplexing leveraging genetic variability

Konrad Hoeft, Tore Bleckwehl, David Schumacher, Hyojin Kim, Robert Meyer, Qingqing Long, Ling Zhang, Christian Möller, Marian C. Clahsen-van Groningen, Anne Babler, Turgay Saritas, Ingo Kurth, Hendrik Milting, Sikander Hayat*, Rafael Kramann*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Single cell RNA sequencing has provided unprecedented insights into the molecular cues and cellular heterogeneity underlying human disease. However, the high costs and complexity of single cell methods remain a major obstacle for generating large-scale human cohorts. Here, we compare current state-of-the-art single cell multiplexing technologies, and provide a widely applicable demultiplexing method, SoupLadle, that enables simple, yet robust high-throughput multiplexing leveraging genetic variability of patients.

Original languageEnglish
Article number10612
JournalNature Communications
Volume15
Issue number1
DOIs
Publication statusPublished - 5 Dec 2024

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Publisher Copyright: © The Author(s) 2024.

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