TY - JOUR
T1 - Label-free single-cell RNA multiplexing leveraging genetic variability
AU - Hoeft, Konrad
AU - Bleckwehl, Tore
AU - Schumacher, David
AU - Kim, Hyojin
AU - Meyer, Robert
AU - Long, Qingqing
AU - Zhang, Ling
AU - Möller, Christian
AU - Clahsen-van Groningen, Marian C.
AU - Babler, Anne
AU - Saritas, Turgay
AU - Kurth, Ingo
AU - Milting, Hendrik
AU - Hayat, Sikander
AU - Kramann, Rafael
N1 - Publisher Copyright: © The Author(s) 2024.
PY - 2024/12/5
Y1 - 2024/12/5
N2 - Single cell RNA sequencing has provided unprecedented insights into the molecular cues and cellular heterogeneity underlying human disease. However, the high costs and complexity of single cell methods remain a major obstacle for generating large-scale human cohorts. Here, we compare current state-of-the-art single cell multiplexing technologies, and provide a widely applicable demultiplexing method, SoupLadle, that enables simple, yet robust high-throughput multiplexing leveraging genetic variability of patients.
AB - Single cell RNA sequencing has provided unprecedented insights into the molecular cues and cellular heterogeneity underlying human disease. However, the high costs and complexity of single cell methods remain a major obstacle for generating large-scale human cohorts. Here, we compare current state-of-the-art single cell multiplexing technologies, and provide a widely applicable demultiplexing method, SoupLadle, that enables simple, yet robust high-throughput multiplexing leveraging genetic variability of patients.
UR - https://www.scopus.com/pages/publications/85211081818
U2 - 10.1038/s41467-024-54270-6
DO - 10.1038/s41467-024-54270-6
M3 - Article
C2 - 39638798
AN - SCOPUS:85211081818
SN - 2041-1723
VL - 15
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 10612
ER -
