Labelling of mammalian cells for visualisation by MRI

Monique Bernsen; AD Moelker; Piotr Wielopolski; Sandra van Tiel; Gabriel Krestin

doi:10.1007/s00330-009-1540-1

Labelling of mammalian cells for visualisation by MRI

Monique Bernsen^*, AD Moelker, Piotr Wielopolski, Sandra van Tiel, Gabriel Krestin

^*Corresponding author for this work

Radiology & Nuclear Medicine

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Through labelling of cells with magnetic contrast agents it is possible to follow the fate of transplanted cells in vivo with magnetic resonance imaging (MRI) as has been demonstrated in animal studies as well as in a clinical setting. A large variety of labelling strategies are available that allow for prolonged and sensitive detection of the labelled cells with MRI. The various protocols each harbour specific advantages and disadvantages. In choosing a particular labelling strategy it is also important to ascertain that the labelling procedure does not negatively influence cell functionality, for which a large variety of assays are available. In order to overcome the challenges still faced in fully exploiting the benefits of in vivo cell tracking by MRI a good understanding and standardisation of the procedures and assays used will be crucial.

Original language	English
Pages (from-to)	255-274
Number of pages	20
Journal	European Radiology
Volume	20
Issue number	2
DOIs	https://doi.org/10.1007/s00330-009-1540-1
Publication status	Published - Feb 2010

Bibliographical note

Funding Information:
The authors would like to acknowledge and thank Dr. Eric Farrell for supplying pictures on differentiated labeled stem cells. This paper has been written in part through support from ENCITE - funded by the European Community under the 7th Framework program.

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Labelling of mammalian cells for visualisation by MRI

Abstract

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