Latent Acyclovir-Resistant Herpes Simplex Virus Type 1 in Trigeminal Ganglia of Immunocompetent Individuals

Monique Velzen; Freek Loenen; Roland Meesters; Miranda de Graaf; Lies Remeijer; Theo Luider; Ab Osterhaus; Georges Verjans

doi:10.1093/infdis/jis237

Latent Acyclovir-Resistant Herpes Simplex Virus Type 1 in Trigeminal Ganglia of Immunocompetent Individuals

Monique Velzen
, Freek Loenen
, Roland Meesters
, Miranda de Graaf
, Lies Remeijer
, Theo Luider
, Ab Osterhaus
, Georges Verjans

Research output: Contribution to journal › Article › Academic › peer-review

39 Citations (Scopus)

Abstract

Specific mutations within the hypervariable herpes simplex virus (HSV) gene thymidine kinase (TK) gene lead to acyclovir (ACV) resistance. To uncover the existence of latent ACV-resistant (ACV(R)) HSV-1, we determined the genetic and functional variability of the HSV-1 TK gene pool in paired trigeminal ganglia (TG) of 5 immunocompetent individuals. The latent virus pool consisted of a donor-specific HSV-1 quasispecies, including one major ACV-sensitive (ACV(S)) and multiple phylogenetic-related minor ACV(S) and ACV(R) TK variants. Contrary to minor variants, major TK variants were shared between paired TG. The data demonstrate the coexistence of phylogenetic-related ACV(S) and ACV(R) latent HSV-1 in human TG.

Original language	Undefined/Unknown
Pages (from-to)	1539-1543
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	205
Issue number	10
DOIs	https://doi.org/10.1093/infdis/jis237
Publication status	Published - 2012

Research programs

EMC MM-03-44-06
EMC MM-04-27-01

Access to Document

10.1093/infdis/jis237

http://hdl.handle.net/1765/39255

Cite this

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title = "Latent Acyclovir-Resistant Herpes Simplex Virus Type 1 in Trigeminal Ganglia of Immunocompetent Individuals",

abstract = "Specific mutations within the hypervariable herpes simplex virus (HSV) gene thymidine kinase (TK) gene lead to acyclovir (ACV) resistance. To uncover the existence of latent ACV-resistant (ACV(R)) HSV-1, we determined the genetic and functional variability of the HSV-1 TK gene pool in paired trigeminal ganglia (TG) of 5 immunocompetent individuals. The latent virus pool consisted of a donor-specific HSV-1 quasispecies, including one major ACV-sensitive (ACV(S)) and multiple phylogenetic-related minor ACV(S) and ACV(R) TK variants. Contrary to minor variants, major TK variants were shared between paired TG. The data demonstrate the coexistence of phylogenetic-related ACV(S) and ACV(R) latent HSV-1 in human TG.",

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T1 - Latent Acyclovir-Resistant Herpes Simplex Virus Type 1 in Trigeminal Ganglia of Immunocompetent Individuals

AU - Velzen, Monique

AU - Loenen, Freek

AU - Meesters, Roland

AU - de Graaf, Miranda

AU - Remeijer, Lies

AU - Luider, Theo

AU - Osterhaus, Ab

AU - Verjans, Georges

PY - 2012

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AB - Specific mutations within the hypervariable herpes simplex virus (HSV) gene thymidine kinase (TK) gene lead to acyclovir (ACV) resistance. To uncover the existence of latent ACV-resistant (ACV(R)) HSV-1, we determined the genetic and functional variability of the HSV-1 TK gene pool in paired trigeminal ganglia (TG) of 5 immunocompetent individuals. The latent virus pool consisted of a donor-specific HSV-1 quasispecies, including one major ACV-sensitive (ACV(S)) and multiple phylogenetic-related minor ACV(S) and ACV(R) TK variants. Contrary to minor variants, major TK variants were shared between paired TG. The data demonstrate the coexistence of phylogenetic-related ACV(S) and ACV(R) latent HSV-1 in human TG.

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DO - 10.1093/infdis/jis237

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