Loss of Expression of the SWI/SNF Chromatin Remodeling Subunit BRG1/SMARCA4 Is Frequently Observed in Intraductal Papillary Mucinous Neoplasms (IPMNs) of the Pancreas

Marco Dal Molin; Seung-Mo Hong; Sachidanand Hebbar; Rajni Sharma; Francesca Scrimieri; Roeland de Wilde; Skye C. Mayo; Michael Goggins; Christopher L. Wolfgang; Richard D. Schulick; Ming-Tseh Lin; James R. Eshleman; Ralph H. Hruban; Anirban Maitra; Hanno Matthaei

Loss of Expression of the SWI/SNF Chromatin Remodeling Subunit BRG1/SMARCA4 Is Frequently Observed in Intraductal Papillary Mucinous Neoplasms (IPMNs) of the Pancreas

Marco Dal Molin, Seung-Mo Hong, Sachidanand Hebbar, Rajni Sharma, Francesca Scrimieri, Roeland de Wilde, Skye C. Mayo, Michael Goggins, Christopher L. Wolfgang, Richard D. Schulick, Ming-Tseh Lin, James R. Eshleman, Ralph H. Hruban, Anirban Maitra, Hanno Matthaei

Research output: Contribution to journal › Meeting Abstract › Academic › peer-review

Abstract

Background: A better molecular characterization of Intraductal Papillary Mucinous Neoplasm (IPMN), the most frequent cystic precursor of pancreatic adenocarcinoma, may have a pivotal role in its early detection and in the development of effective therapeutic strategies. BRG1, a central component of the chromatin remodeling complex SWI/SNF regulating transcription, is inactive in several malignancies.

Methods: We evaluated Brg1 expression in IPMN in order to better understand its role in pancreatic carcinogenesis. Tissue microarrays (TMAs) of 66 surgically resected IPMNs were immunolabeled for the Brg1 protein. Expression patterns were correlated with clinicopathologic parameters.

Results: Normal pancreatic epithelium strongly immunolabeled for Brg1. Reduced Brg1 expression was observed in 32 (53.3%) of the 60 evaluable lesions and occurred more frequently in high-grade IPMNs (13 of 17 showed loss; 76%) compared to intermediate-grade (15 of 29 showed loss; 52%) and low-grade (4 of 14 showed loss; 28%) (p=0.03). A complete loss of expression was observed in 5 of the 60 (8.3%) lesions. Finally, a decrease in Brg1 protein expression was found in a low-passage non-invasive IPMN cell line by Western blot analysis.

Conclusion: We provide first evidence that Brg1 expression is lost in IPMN.

Original language	English
Pages (from-to)	1318-1319
Number of pages	2
Journal	Pancreas
Volume	40
Issue number	8
Publication status	Published - Nov 2011
Externally published	Yes

Cite this

Dal Molin, M., Hong, S.-M., Hebbar, S., Sharma, R., Scrimieri, F., de Wilde, R., Mayo, S. C., Goggins, M., Wolfgang, C. L., Schulick, R. D., Lin, M.-T., Eshleman, J. R., Hruban, R. H., Maitra, A., & Matthaei, H. (2011). Loss of Expression of the SWI/SNF Chromatin Remodeling Subunit BRG1/SMARCA4 Is Frequently Observed in Intraductal Papillary Mucinous Neoplasms (IPMNs) of the Pancreas. Pancreas, 40(8), 1318-1319.

@article{3e86ecd224554df297f01e57a973835f,

title = "Loss of Expression of the SWI/SNF Chromatin Remodeling Subunit BRG1/SMARCA4 Is Frequently Observed in Intraductal Papillary Mucinous Neoplasms (IPMNs) of the Pancreas",

abstract = "Background: A better molecular characterization of Intraductal Papillary Mucinous Neoplasm (IPMN), the most frequent cystic precursor of pancreatic adenocarcinoma, may have a pivotal role in its early detection and in the development of effective therapeutic strategies. BRG1, a central component of the chromatin remodeling complex SWI/SNF regulating transcription, is inactive in several malignancies. Methods: We evaluated Brg1 expression in IPMN in order to better understand its role in pancreatic carcinogenesis. Tissue microarrays (TMAs) of 66 surgically resected IPMNs were immunolabeled for the Brg1 protein. Expression patterns were correlated with clinicopathologic parameters. Results: Normal pancreatic epithelium strongly immunolabeled for Brg1. Reduced Brg1 expression was observed in 32 (53.3\%) of the 60 evaluable lesions and occurred more frequently in high-grade IPMNs (13 of 17 showed loss; 76\%) compared to intermediate-grade (15 of 29 showed loss; 52\%) and low-grade (4 of 14 showed loss; 28\%) (p=0.03). A complete loss of expression was observed in 5 of the 60 (8.3\%) lesions. Finally, a decrease in Brg1 protein expression was found in a low-passage non-invasive IPMN cell line by Western blot analysis. Conclusion: We provide first evidence that Brg1 expression is lost in IPMN.",

author = "\{Dal Molin\}, Marco and Seung-Mo Hong and Sachidanand Hebbar and Rajni Sharma and Francesca Scrimieri and \{de Wilde\}, Roeland and Mayo, \{Skye C.\} and Michael Goggins and Wolfgang, \{Christopher L.\} and Schulick, \{Richard D.\} and Ming-Tseh Lin and Eshleman, \{James R.\} and Hruban, \{Ralph H.\} and Anirban Maitra and Hanno Matthaei",

year = "2011",

month = nov,

language = "English",

volume = "40",

pages = "1318--1319",

journal = "Pancreas",

issn = "0885-3177",

publisher = "Lippincott Williams \& Wilkins",

number = "8",

}

Dal Molin, M, Hong, S-M, Hebbar, S, Sharma, R, Scrimieri, F, de Wilde, R, Mayo, SC, Goggins, M, Wolfgang, CL, Schulick, RD, Lin, M-T, Eshleman, JR, Hruban, RH, Maitra, A & Matthaei, H 2011, 'Loss of Expression of the SWI/SNF Chromatin Remodeling Subunit BRG1/SMARCA4 Is Frequently Observed in Intraductal Papillary Mucinous Neoplasms (IPMNs) of the Pancreas', Pancreas, vol. 40, no. 8, pp. 1318-1319.

Loss of Expression of the SWI/SNF Chromatin Remodeling Subunit BRG1/SMARCA4 Is Frequently Observed in Intraductal Papillary Mucinous Neoplasms (IPMNs) of the Pancreas. / Dal Molin, Marco; Hong, Seung-Mo; Hebbar, Sachidanand et al.
In: Pancreas, Vol. 40, No. 8, 11.2011, p. 1318-1319.

Research output: Contribution to journal › Meeting Abstract › Academic › peer-review

TY - JOUR

T1 - Loss of Expression of the SWI/SNF Chromatin Remodeling Subunit BRG1/SMARCA4 Is Frequently Observed in Intraductal Papillary Mucinous Neoplasms (IPMNs) of the Pancreas

AU - Dal Molin, Marco

AU - Hong, Seung-Mo

AU - Hebbar, Sachidanand

AU - Sharma, Rajni

AU - Scrimieri, Francesca

AU - de Wilde, Roeland

AU - Mayo, Skye C.

AU - Goggins, Michael

AU - Wolfgang, Christopher L.

AU - Schulick, Richard D.

AU - Lin, Ming-Tseh

AU - Eshleman, James R.

AU - Hruban, Ralph H.

AU - Maitra, Anirban

AU - Matthaei, Hanno

PY - 2011/11

Y1 - 2011/11

N2 - Background: A better molecular characterization of Intraductal Papillary Mucinous Neoplasm (IPMN), the most frequent cystic precursor of pancreatic adenocarcinoma, may have a pivotal role in its early detection and in the development of effective therapeutic strategies. BRG1, a central component of the chromatin remodeling complex SWI/SNF regulating transcription, is inactive in several malignancies. Methods: We evaluated Brg1 expression in IPMN in order to better understand its role in pancreatic carcinogenesis. Tissue microarrays (TMAs) of 66 surgically resected IPMNs were immunolabeled for the Brg1 protein. Expression patterns were correlated with clinicopathologic parameters. Results: Normal pancreatic epithelium strongly immunolabeled for Brg1. Reduced Brg1 expression was observed in 32 (53.3%) of the 60 evaluable lesions and occurred more frequently in high-grade IPMNs (13 of 17 showed loss; 76%) compared to intermediate-grade (15 of 29 showed loss; 52%) and low-grade (4 of 14 showed loss; 28%) (p=0.03). A complete loss of expression was observed in 5 of the 60 (8.3%) lesions. Finally, a decrease in Brg1 protein expression was found in a low-passage non-invasive IPMN cell line by Western blot analysis. Conclusion: We provide first evidence that Brg1 expression is lost in IPMN.

AB - Background: A better molecular characterization of Intraductal Papillary Mucinous Neoplasm (IPMN), the most frequent cystic precursor of pancreatic adenocarcinoma, may have a pivotal role in its early detection and in the development of effective therapeutic strategies. BRG1, a central component of the chromatin remodeling complex SWI/SNF regulating transcription, is inactive in several malignancies. Methods: We evaluated Brg1 expression in IPMN in order to better understand its role in pancreatic carcinogenesis. Tissue microarrays (TMAs) of 66 surgically resected IPMNs were immunolabeled for the Brg1 protein. Expression patterns were correlated with clinicopathologic parameters. Results: Normal pancreatic epithelium strongly immunolabeled for Brg1. Reduced Brg1 expression was observed in 32 (53.3%) of the 60 evaluable lesions and occurred more frequently in high-grade IPMNs (13 of 17 showed loss; 76%) compared to intermediate-grade (15 of 29 showed loss; 52%) and low-grade (4 of 14 showed loss; 28%) (p=0.03). A complete loss of expression was observed in 5 of the 60 (8.3%) lesions. Finally, a decrease in Brg1 protein expression was found in a low-passage non-invasive IPMN cell line by Western blot analysis. Conclusion: We provide first evidence that Brg1 expression is lost in IPMN.

UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=eur_pure&SrcAuth=WosAPI&KeyUT=WOS:000296398000073&DestLinkType=FullRecord&DestApp=WOS

UR - https://journals.lww.com/pancreasjournal/toc/2011/11000

UR - https://journals.lww.com/pancreasjournal/fulltext/2011/11000/abstracts_of_papers_submitted_to_the_42nd_annual.26.aspx

M3 - Meeting Abstract

SN - 0885-3177

VL - 40

SP - 1318

EP - 1319

JO - Pancreas

JF - Pancreas

IS - 8

ER -

Loss of Expression of the SWI/SNF Chromatin Remodeling Subunit BRG1/SMARCA4 Is Frequently Observed in Intraductal Papillary Mucinous Neoplasms (IPMNs) of the Pancreas

Abstract

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Loss of expression of the SWI/SNF chromatin remodeling subunit BRG1/SMARCA4 is frequently observed in intraductal papillary mucinous neoplasms of the pancreas

Cite this