Low Input Targeted Chromatin Capture (Low-T2C)

Ilias Boltsis; Karol Nowosad; Rutger W.W. Brouwer; Przemko Tylzanowski; Wilfred F.J. van IJcken; Danny Huylebroeck; Frank Grosveld; Petros Kolovos

doi:10.1007/978-1-0716-1597-3_9

Low Input Targeted Chromatin Capture (Low-T2C)

Ilias Boltsis, Karol Nowosad, Rutger W.W. Brouwer, Przemko Tylzanowski, Wilfred F.J. van IJcken, Danny Huylebroeck, Frank Grosveld^*, Petros Kolovos

^*Corresponding author for this work

Cell biology

Research output: Chapter/Conference proceeding › Chapter › Academic

Abstract

Targeted chromatin capture (T2C) is a 3C-based method and is used to study the 3D chromatin organization, interactomes and structural changes associated with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinformatics tools in detail.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	165-179
Number of pages	15
DOIs	https://doi.org/10.1007/978-1-0716-1597-3_9
Publication status	Published - 2021

Publication series

Series	Methods in Molecular Biology
Volume	2351
ISSN	1064-3745

Bibliographical note

Funding Information:
We would also like to thank the members of Huylebroeck and Grosveld laboratories for the insightful comments and helpful discussions, and the members of the Center for Biomics at Erasmus Medical Center for their technical assistance. IB was supported by NWO. PT and KN are partially supported by the UMO-2015/19/ B/NZ4/03184 grant.

Publisher Copyright:
© 2021, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Cite this

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AU - van IJcken, Wilfred F.J.

AU - Huylebroeck, Danny

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AU - Kolovos, Petros

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Low Input Targeted Chromatin Capture (Low-T2C)

Abstract

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