Low Input Targeted Chromatin Capture (Low-T2C)

Ilias Boltsis, Karol Nowosad, Rutger W.W. Brouwer, Przemko Tylzanowski, Wilfred F.J. van IJcken, Danny Huylebroeck, Frank Grosveld*, Petros Kolovos

*Corresponding author for this work

Research output: Chapter/Conference proceedingChapterAcademic


Targeted chromatin capture (T2C) is a 3C-based method and is used to study the 3D chromatin organization, interactomes and structural changes associated with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinformatics tools in detail.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages15
Publication statusPublished - 2021

Publication series

SeriesMethods in Molecular Biology

Bibliographical note

Funding Information:
We would also like to thank the members of Huylebroeck and Grosveld laboratories for the insightful comments and helpful discussions, and the members of the Center for Biomics at Erasmus Medical Center for their technical assistance. IB was supported by NWO. PT and KN are partially supported by the UMO-2015/19/ B/NZ4/03184 grant.

Publisher Copyright:
© 2021, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


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