TY - CHAP
T1 - Luminescent probes and visualization of bioluminescence
AU - Michelini, Elisa
AU - Cevenini, Luca
AU - Mezzanotte, Laura
AU - Roda, Aldo
PY - 2009
Y1 - 2009
N2 - Bioluminescence (BL) has revealed an extremely useful analytical tool enabling ultrasensitive detection in biotechnological applications. Following ohe discovery of luciferin and luciferases, molecular biology techniques allowed the cloning of several luciferases and photoproteins. Among most used BL reporters, we find firefly and click-beetle luciferases, bacterial luciferase, Renilla, Gaussia, and Cypridina luciferases, and calcium-activated photoproteins. According to the specific bioluminescent protein, different substrates and protocols must be applied in the experimental procedure for BL measurement. By conjugating (either chemically or by molecular biology techniques) bioluminescent probes to specific targets, it is in fact possible to track a wide range of events and analytes. To aid investigators in the choice and applications of reporter genes, the materials and methods required for BL measurements and experimental protocols are described.
AB - Bioluminescence (BL) has revealed an extremely useful analytical tool enabling ultrasensitive detection in biotechnological applications. Following ohe discovery of luciferin and luciferases, molecular biology techniques allowed the cloning of several luciferases and photoproteins. Among most used BL reporters, we find firefly and click-beetle luciferases, bacterial luciferase, Renilla, Gaussia, and Cypridina luciferases, and calcium-activated photoproteins. According to the specific bioluminescent protein, different substrates and protocols must be applied in the experimental procedure for BL measurement. By conjugating (either chemically or by molecular biology techniques) bioluminescent probes to specific targets, it is in fact possible to track a wide range of events and analytes. To aid investigators in the choice and applications of reporter genes, the materials and methods required for BL measurements and experimental protocols are described.
UR - https://www.scopus.com/pages/publications/84934437733
U2 - 10.1007/978-1-60327-321-3_1
DO - 10.1007/978-1-60327-321-3_1
M3 - Chapter
C2 - 19685295
AN - SCOPUS:84934437733
SN - 9781603273206
T3 - Methods in Molecular Biology
SP - 1
EP - 13
BT - Bioluminescence
ER -